Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20)

CHEN Guo-lin; et al

您当前的位置：

首页 >

文章列表页 >

Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20)

更新时间：2024-01-17

- Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20)
- Journal of Chongqing Medical University Issue 11, Pages: 1338-1340(2008)
- 作者机构：
  
  1. 哈尔滨医科大学附属第一医院感染科
  2. 重庆医科大学病毒性肝炎研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.7
- Published：2008，
扫描看全文
[1]陈国林,胡怀东,袁迪,李用国,任红,马英骥.CIK细胞对裸鼠人肝癌转移预防和治疗[J].重庆医科大学学报,2008(11):1338-1340.

CHEN Guo-lin, et al. Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20). [J]. Journal of Chongqing Medical University (11):1338-1340(2008)
[1]陈国林,胡怀东,袁迪,李用国,任红,马英骥.CIK细胞对裸鼠人肝癌转移预防和治疗[J].重庆医科大学学报,2008(11):1338-1340. DOI：

CHEN Guo-lin, et al. Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20). [J]. Journal of Chongqing Medical University (11):1338-1340(2008) DOI：

摘要

目的:探讨人细胞因子杀伤（Cytokine-inducedkiller

CIK）细胞对裸鼠人肝癌转移模型（LCI-D20）治疗作用。方法:用淋巴细胞分离液分离外周血单个核细胞

加入细胞因子rhIFN-γ、人CD3mAb和rhIL-2

诱导生成CIK细胞

经腹腔注射

同时应用PBS作为对照

研究其对LCI-D20肝癌治疗和预防转移作用。结果:CIK组和对照组肝癌肿块重量、血AFP含量、肝癌肝内转移率和生存期依次分别为（1.125±0.363）g和（2.682±0.526）g;（48.6±5.2）μg/L和（82.2±7.2）μg/L;16.7%和58.3%;（73.3±7.03）d和（52.3±5.24）d

与对照组比较均P<0.01。结论:CIK细胞可以预防LCI-D20模型肝癌发生转移

延长动物生存时间。

Abstract

Objective:To investigate the cure effect of human cytokine-induced killer (CIK) cells on the model of human hepatocellular carcinoma in nude mice. Methods:Peripheral blood mononuclear cells(PBMC)were obtained from healthy volun- teers by Ficoll gradient centrifugation and CIK cells were induced by culturing PBMC in the completed medium containing rhIFN-γ

rhIL-2 and mAb against CD3.By intraperitoneal injection of CIK cells in LCI-D20

the effect of prevention and therapy to HCC in LCI-D20 were assessed. Results:The weight of tumor in the CIK cells group and negative group was (1.125±0.363)gram and(2.682±0.526)gram respectively(t=6.06

P<0.01). The amount of AFP in blood of the group of CIK cells was(48.6±5.2)μg/L as the amount in the negative group was (82.2±7.2)μg/L(t=22.07

P<0.01). The metastasis rate in liver was 16.7% and 58.3% between two groups(χ2=4.44

P<0.01). The survival time of model in the therapy group were(73.3±7.03) days in the CIK group as compared with (52.3±5.24)days in negative group

(t=17.54

P<0.01). Conclusion:CIK cells may prevent metastasis in the model of LCI-D20 and prolong the survival time.

关键词

细胞因子杀伤细胞癌肝细胞

Keywords

Cytokine-induced killer cellscarcinomaHepatocellular

references

Views

104

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

HORIZONTAL GLOTTECTOMY FOR CARCINOMA OF LARYNX INVOLVING BOTH VOCAL CORDS

MODIFIED HORIZONTAL GLOTTECTOMY FOR TREATING THE CANCER OF BILATERAL VOCAL CORDS

Proteomic analysis of esophageal squamous cell carcinoma by laser capture microdissection

Clinical analysis of differential diagnosis between renal cell carcinoma and renal benign space occupying mass

Clinical results of chemotherapy applying docetaxel-cisplatin combination for advanced hormone independent prostate cancer

Related Author

周道芬

黄征丽

项菊芬

陈佩君

周建荣

LI Juncai

et al

Related Institution

重庆医科大学第一医院耳鼻咽喉科

重庆医科大学第一医院耳鼻喉科

Department of General Surgery,the First Affiliated Hospital,Chongqing Medical University

内分泌科重庆医科大学附属第一医院

重庆医科大学基础医学研究所

⁰