Effects of vitamin A deficiency during pregnancy on the lung vitamin A status,its receptor and pulmonary surfactant of fetal rats

LIU You-hong; et al

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Effects of vitamin A deficiency during pregnancy on the lung vitamin A status,its receptor and pulmonary surfactant of fetal rats

更新时间：2024-01-17

- Effects of vitamin A deficiency during pregnancy on the lung vitamin A status,its receptor and pulmonary surfactant of fetal rats
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 728-731(2009)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院新生儿科
  2. 广州市儿童医院
  3. 重庆医科大学附属儿童医院儿童营养研究中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R714.2
- Published：2009，
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[1]刘友红,陈贻骥,房春晓,瞿平.孕鼠VA缺乏对胎肺VA及其受体和表面活性蛋白基因的影响[J].重庆医科大学学报,2009,34(06):728-731.

LIU You-hong, et al. Effects of vitamin A deficiency during pregnancy on the lung vitamin A status,its receptor and pulmonary surfactant of fetal rats. [J]. Journal of Chongqing Medical University 34(6):728-731(2009)
[1]刘友红,陈贻骥,房春晓,瞿平.孕鼠VA缺乏对胎肺VA及其受体和表面活性蛋白基因的影响[J].重庆医科大学学报,2009,34(06):728-731. DOI：

LIU You-hong, et al. Effects of vitamin A deficiency during pregnancy on the lung vitamin A status,its receptor and pulmonary surfactant of fetal rats. [J]. Journal of Chongqing Medical University 34(6):728-731(2009) DOI：

摘要

目的:探讨孕鼠维生素A（Vitamin A

VA）缺乏对胎肺VA及其受体和表面活性蛋白基因的影响。方法:建立孕期VA正常（Vitamin A normal

VAN）和亚临床缺乏（Marginal vitamin A deficiency

MVAD）动物模型各6只

每组再随机分为地塞米松（Dexamethasone

Dex）组和生理盐水（Normal saline

NS）组

即VAN-Dex组、VAN-NS组、MVAD-Dex组和MVAD-NS组。各组均于孕16、17、18d肌肉注射Dex或NS

于孕19d剖宫取胎鼠

高效液相色谱法（High performance liquid chromatography

HPLC）检测胎鼠肺组织VA含量

逆转录-聚合酶链反应（Reverse transcription-polymerase chain reaction

RT-PCR）方法检测胎鼠肺表面活性蛋白（Pulmonary surfactant

PS）和视黄酸受体（Retinoic acid receptor

RAR）mRNAs的表达

以甘油醛-3-磷酸脱氢酶（Glyc-eraldehyde-3-phosphate dehydrogenase

GAPDH）mRNA扩增产物为内参照

密度扫描作半定量分析。结果:（1）胎鼠肺单位组织VA水平:VAN-Dex组>VAN-NS组

MVAD-Dex组>MVAD-NS组

VAN-Dex组>MVAD-Dex组

VAN-NS组>MVAD-NS组

各组比较差异均有统计学意义（P<0.05）。（2）SP-B和SP-CmRNAs的表达水平:VAN-NS组>MVAD-NS组

MVAD-Dex组>MVAD-NS组

比较差异均有统计学意义（P<0.05）;VAN-Dex组>VAN-NS组

VAN-Dex组>MVAD-Dex组

但比较差异均无统计学意义（P>0.05）。（3）RAR-α、RAR-γ和RAR-βmRNAs表达水平:VAN-Dex组>VAN-NS组

MVAD-Dex组>MVAD-NS组

VAN-Dex组<MVAD-Dex组

VAN-NS组<MVAD-NS组

3个基因的表达水平各组比较差异均有统计学意义（P<0.05）。结论:孕期VA缺乏能不同程度的影响胎鼠肺单位组织VA水平、SP-B和SP-CmRNAs以及RAR-α、RAR-γ和RAR-βmRNAs的表达;孕期MVAD时其胎肺成熟度较低

但不影响Dex促胎肺成熟作用的发挥。

Abstract

Objective:To observe the effects of vitamin A deficiency during pregnancy on the lung vitamin A status

its receptor and pulmonary surfactant of fetal rats.Methods:Prenatal vitamin A normal(VAN)and marginal vitamin A deficiency(MVAD)of Sprague-Dawley rat models were employed.There were 6 in each group.Then they were randomly divided into dexamethasone(Dex)group and control group respectively.I.e VAN-Dex group

VAN-NS group

MVAD-Dex group and MVAD-NS group.From the gestational days 16 to 18

all the rats were given Dex or Saline by intramuscular injection respectively.On the gestational day 19

fetal rats were harvested by cesarean section.The vitamin A concentrations of lung were detected by high-performance liquid chromatography(HPLC)

and the levels of pulmonary surfactant(PS)and retinoic acid receptor(RAR)mRNAs were detected by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR);then the values of PS and RAR mRNAs were compared to glyceraldehyde-3-phosphate dehydrogenase(GAPDH)mRNA.Results:(1)The vitamin A concentrations of lungs in VAN-NS group were higher than those of MVAD-NS group

higher in VAN-Dex group than in MVAD-Dex group

higher in MVAD-Dex group than in MVAD-NS group

and higher in VAN-Dex group than in VAN-NS group.There were significant differences among the four groups(P<0.05);(2)As to the levels of SP-B and SP-C mRNAs

they were higher in VAN-NS group than those in MVAD-NS group

and higher in MVAD-Dex group than in MVAD-NS group.There were significant differences between the two groups(P<0.05).The levels in VAN-Dex group were higher than those in VAN-NS group

higher in VAN-Dex group than in MVAD-Dex group

but there were no significant differences(P>0.05).(3)With regard to the levels of RAR mRNA

the levels of RAR-α and RAR-γ mRNAs in VAN-Dex group were higher than those in VAN-NS group

higher in MVAD-Dex group than in MVAD-NS group

lower in VAN-Dex group than in MVAD-Dex group

lower in VAN-NS group than in MVAD-NS group.The levels of RAR-β mRNA in the four groups were similar to those of the RAR-α and RAR-γ mRNAs.And There were significant differences(P<0.05).Conclusion:The vitamin A concentrations of lungs and the levels of SP-B and SP-C mRNAs

the levels of RAR mRNA

were all influenced by vitamin A deficiency during pregnancy.The fetal lungs of the MVAD’s mother is immature

but the effects of Dex will not be affected.

关键词

维生素A表面活性蛋白视黄酸受体胎鼠

Keywords

vitamin APulmonary surfactantRetinoic acid receptorFetal rats

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