Zileuton,a 5-LOX inhibitor,inhibits proliferation and induces apoptos is of SGC-7901 gastric cancer cells

PAN Min; et al

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Zileuton,a 5-LOX inhibitor,inhibits proliferation and induces apoptos is of SGC-7901 gastric cancer cells

更新时间：2024-01-17

- Zileuton,a 5-LOX inhibitor,inhibits proliferation and induces apoptos is of SGC-7901 gastric cancer cells
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 562-566(2009)
- 作者机构：
  
  重庆医科大学附属第一医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.2
- Published：2009，
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[1]潘敏,刘纯伦,方晓杰,石建华.齐留通对胃癌SGC-7901细胞生长的影响[J].重庆医科大学学报,2009,34(05):562-566.

PAN Min, et al. Zileuton,a 5-LOX inhibitor,inhibits proliferation and induces apoptos is of SGC-7901 gastric cancer cells. [J]. Journal of Chongqing Medical University 34(5):562-566(2009)
[1]潘敏,刘纯伦,方晓杰,石建华.齐留通对胃癌SGC-7901细胞生长的影响[J].重庆医科大学学报,2009,34(05):562-566. DOI：

PAN Min, et al. Zileuton,a 5-LOX inhibitor,inhibits proliferation and induces apoptos is of SGC-7901 gastric cancer cells. [J]. Journal of Chongqing Medical University 34(5):562-566(2009) DOI：

摘要

目的:观察5-脂氧合酶抑制剂齐留通对胃癌SGC-7901细胞增殖、凋亡的影响

初步探讨齐留通影响胃癌细胞生长的机制。方法:MTT法测定齐留通对胃癌SGC-7901细胞增殖的影响;TUNEL染色法计数细胞凋亡率;RT-PCR和免疫细胞化学检测齐留通处理前后SGC-7901细胞Bax、Bcl-2和caspase-3 mRNA和其蛋白表达的改变。结果:齐留通以时间剂量依赖的方式抑制SGC-7901细胞生长;TUNEL染色法显示6.2×10-5mol/L、3.1×10-5mol/L齐留通作用72h后细胞凋亡率为19.6%±6.3%和28.9%±2.3%

均显著高于对照组0.6%±0.1%（P<0.01）;RT-PCR和免疫细胞化学显示6.2×10-5mol/L齐留通作用胃癌SGC-7901细胞72h后

可显著促进Bax、caspase-3 mRNA和其蛋白的表达（P<0.05）

抑制Bcl-2 mRNA及其蛋白的表达（P<0.05）。结论:齐留通能抑制胃癌SGC-7901细胞增殖

并通过促进Bax和caspase-3的表达

抑制Bcl-2表达

诱导其凋亡

从而抑制胃癌SGC-7901细胞生长。

Abstract

Objective:To evaluate the efficacy of 5-LOX inhibitor zileuton on the proliferation and apoptosis of human gastric carcinoma cell line SGC-7901

and to explore the mechanisms of apoptosis induced by zileuton.Methods:The proliferation of SGC-7901 cells was detected by thiazolyl blue tetrazolium bromide(MTT)assays.TUNEL staining was used to evaluate the apoptosis of SGC-7901 cells.The changes of Bax

Bcl-2 and caspase-3 mRNA expression were studied by reverse transcription polymerase chain reaction.Bax

Bcl-2 and caspase-3 protein expression were analyzed by immunocytochemistry in SGC-7901 cells treated with zileuton.Results:Zileuton can decrease the proliferation significantly in a dose-dependent and time-dependent manner in SGC-7901 cells.TUNEL staining showed that the apoptosis rate was elevated with the increase of zileuton concentration(P<0.01).6.2×10-5 mol/L zileuton effectively inhibited the mRNA and protein expression of Bcl-2(P<0.05)

but increased Bax and caspase-3 expression(P<0.05).Conclusion:5 LOX inhibitor zileuton can inhibit proliferation and induce apoptosis of SGC-7901 cells by down regulating the expression of Bcl-2 and up regulating Bax and caspase-3.

关键词

齐留通胃癌SGC-7901细胞5-脂氧合酶抑制剂凋亡

Keywords

Zileutongastric cancerSGC-7901 cells5-LOX inhibitorapoptosis

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Related Institution

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

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Department of General Surgery，the First Affiliated Hospital，Chongqing Medical University

Teaching and Research Section of Pathophysiology，College of Basic Medicine，Chongqing Medical University

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