Effects of antisense oligonucleotide targeting hsa-miR-518b on the growth of gastric cancer cell

LIU Bo; et al

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Effects of antisense oligonucleotide targeting hsa-miR-518b on the growth of gastric cancer cell

更新时间：2024-01-17

- Effects of antisense oligonucleotide targeting hsa-miR-518b on the growth of gastric cancer cell
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 567-569(2009)
- 作者机构：
  
  重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.2
- Published：2009，
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[1]刘波,吴小翎,聂娜.hsa-miR-518b反义寡核苷酸对胃癌细胞生长抑制作用的研究[J].重庆医科大学学报,2009,34(05):567-569.

LIU Bo, et al. Effects of antisense oligonucleotide targeting hsa-miR-518b on the growth of gastric cancer cell. [J]. Journal of Chongqing Medical University 34(5):567-569(2009)
[1]刘波,吴小翎,聂娜.hsa-miR-518b反义寡核苷酸对胃癌细胞生长抑制作用的研究[J].重庆医科大学学报,2009,34(05):567-569. DOI：

LIU Bo, et al. Effects of antisense oligonucleotide targeting hsa-miR-518b on the growth of gastric cancer cell. [J]. Journal of Chongqing Medical University 34(5):567-569(2009) DOI：

摘要

目的:探讨hsa-miR-518b反义寡核苷酸（Antisense oligonucleotide

ASODN）对胃癌细胞生长和细胞周期的影响

以分析其在胃癌中的作用机制。方法:设计合成全硫代磷酸化修饰的hsa-miR-518b ASODN

将其转染于胃癌细胞株SGC7901。用Realtime RT-PCR分析hsa-miR-518b在胃癌细胞转染前后的表达情况

MTT检测胃癌细胞转染后的生长情况

流式细胞仪分析细胞周期。结果:转染hsa-miR-518b ASODN后

胃癌细胞hsa-miR-518b表达显著降低（P=0.000）

生长受到抑制（P=0.000）

大量胃癌细胞阻滞于G1期（P=0.000）。结论:hsa-miR-518b ASODN能使胃癌细胞阻滞于G1期

抑制胃癌细胞的生长。提示hsa-miR-518b可能通过促进细胞周期G1/S期转换而促进胃癌细胞生长。

Abstract

Objective:Toinvestigate the effects ofantisense oligonucleotide(ASODN)targetinghsa-miR-518b on the growth and cycle of gastric cancer cell

and the mechanism in gastric cancer.Methods:Specific phosphorothioate antisense oligodeoxynucleotides targeting hsa-miR-518b were synthesized and then transfected into the gastric cancer cel(lSGC7901).We investigated the change of the hsa-miR-518b expression in SGC7901 after the transfection by using real time RT-PCR.Methyl thiazolyl tetrazolium(MTT)assay was used to detect the cell growth of SGC 7901 and fluorescence activated cell sorting(FACS)was used to analyze the cell cycle of SGC7901.Results:After transfection

the expression of hsa-miR-518b was decrased(P=0.000)

the growth of SGC7901 was suppressed(P=0.000) and the cell cycle arrested in G(1P=0.000).Conclusion:hsa-miR-518b(ASODN)could suppress the growth of SGC 7901 by arresting the cell cycle in G1 phase.Hsa-miR-518b could promote the growth of gastric cancer cell by regulating Gl/S transition.

关键词

hsa-miR-518b胃癌细胞反义寡核苷酸

Keywords

Has-miR-518bGastric cancer cellAntisense oligonucleotides

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