Improvement of primary culture of cardiac myocytes in neonatal rat

WANG Jia-nan; et al

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Improvement of primary culture of cardiac myocytes in neonatal rat

更新时间：2024-01-17

- Improvement of primary culture of cardiac myocytes in neonatal rat
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 600-603(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院心内科
  2. 重庆医科大学病理生理学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q813.11
- Published：2009，
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[1]王佳南,张晓刚,汤为学,郑丽娜.新生大鼠心肌细胞原代培养方法的改良[J].重庆医科大学学报,2009,34(05):600-603.

WANG Jia-nan, et al. Improvement of primary culture of cardiac myocytes in neonatal rat. [J]. Journal of Chongqing Medical University 34(5):600-603(2009)
[1]王佳南,张晓刚,汤为学,郑丽娜.新生大鼠心肌细胞原代培养方法的改良[J].重庆医科大学学报,2009,34(05):600-603. DOI：

WANG Jia-nan, et al. Improvement of primary culture of cardiac myocytes in neonatal rat. [J]. Journal of Chongqing Medical University 34(5):600-603(2009) DOI：

摘要

目的:探讨更为简便的新生大鼠心肌细胞原代培养的方法

使分离的心肌细胞达到理想的数量、存活率和纯度。方法:用胰蛋白酶和Ⅱ型胶原酶联合消化

分离新生大鼠心肌细胞

进行体外培养

观察心肌细胞形态学变化

并以细胞免疫荧光进行心肌细胞纯度鉴定。结果:心肌细胞分离良好

收获细胞数量及存活率高

可贴壁搏动生长

免疫荧光显示培养的心肌细胞纯度达95%以上。结论:改良的细胞培养技术可获得生长状态良好的心肌细胞。

Abstract

Objective:To discuss how the more convenient primary culture method of rats’cardiac myocytes to increase the quantity

livability and purity of isolated cells.Methods:Neonatal rats’cardiac myocytes were isolated by co-digestion of trypsin and collagenase Ⅱ

and then cultured in vitro.Morphocytology changes were observed and cell purity was evaluated by immunofluorescence.Results:Isolation of cardiomyocytes was with a good quantity and high livability.Cells adhered and impulsed well.Immunofluorescence staining confirmed the cell with purity higher than 95%.Conclusion:The modified cell culture method can obtain the Cardiomyocytes with good growth state.

关键词

心肌细胞原代培养新生大鼠

Keywords

Cardiac myocyteprimary cultureneonatal rat

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