Simvastatin prevents rat cardiac hypertrophy induced by myocardial infarction via RhoA kinase

HUANG Ji-wei; et al

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Simvastatin prevents rat cardiac hypertrophy induced by myocardial infarction via RhoA kinase

更新时间：2024-01-17

- Simvastatin prevents rat cardiac hypertrophy induced by myocardial infarction via RhoA kinase
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 164-167(2009)
- 作者机构：
  
  1. 四川省自贡市第一人民医院心内科
  2. 重庆医科大学附属第一医院心内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R542.22;R96
- Published：2009，
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[1]黄纪卫,覃数,李向东,张冬颖.辛伐他汀抑制心肌梗死后大鼠心肌细胞肥大与RhoA激酶关系[J].重庆医科大学学报,2009,34(02):164-167.

HUANG Ji-wei, et al. Simvastatin prevents rat cardiac hypertrophy induced by myocardial infarction via RhoA kinase. [J]. Journal of Chongqing Medical University 34(2):164-167(2009)
[1]黄纪卫,覃数,李向东,张冬颖.辛伐他汀抑制心肌梗死后大鼠心肌细胞肥大与RhoA激酶关系[J].重庆医科大学学报,2009,34(02):164-167. DOI：

HUANG Ji-wei, et al. Simvastatin prevents rat cardiac hypertrophy induced by myocardial infarction via RhoA kinase. [J]. Journal of Chongqing Medical University 34(2):164-167(2009) DOI：

摘要

目的:观察辛伐他汀对心肌梗死后大鼠心肌细胞肥大的抑制作用

探讨小G蛋白RhoA激酶（RhoA kinase

ROCK）在辛伐他汀调控心肌细胞肥大中的信号转导作用。方法:雄性Wistar大鼠

分为4组

每组8只。结扎冠状动脉左前降支

建立心肌梗死模型。假手术组（SHAM组）和梗死组（MI组）予生理盐水1ml/d灌胃。辛伐他汀组（SIM组）予辛伐他汀40mg（/kg.d）灌胃。Fasudil组（F组）予特异性ROCK抑制剂Fasudil 7.5mg/kg

bid腹腔注射。术后28d

测定大鼠左室重量指数

心肌细胞横断面积

RT-PCR检测ROCKmRNA表达

免疫组化和Western-Blot检测ROCK活性标志物-磷酸化ERM（Phospho-ERM）蛋白表达水平。结果:术后28d

MI组大鼠心肌左室重量指数

心肌细胞表面积

ROCK mRNA表达水平以及Phospho-ERM蛋白表达水平较SHAM组明显增高

SIM组和F组前述各项检测指标均较MI组显著降低。结论:辛伐他汀能抑制心梗后心肌细胞肥大

其作用机制可能与他汀抑制ROCK途径有关。

Abstract

Objective:To study the inhibition effects of simvastatin on cardiac hypertrophy induced by myocardial infarction

and to in-vestigate whether simvastatin prevents cardiac hypertrophy through attenuation of RhoA kinase.Methods:Male Wistar rats were divided intofourgroup(sn=8).Myocardial infarction model was established by ligation of left anterior descending coronary artery.Sham operati on group(SHAM group)and myocardidal infarction group(MI group)were gavaged with 0.9%NaCl 1ml/day

Simvastatin group(SIM group)was gavaged with simvastatin 40 mg/(kg.d).Fasudil group(F group)was injected with fasudil 7.5mg(/kg.bid)through peritoneum.At the time point of 28 ds

the ratio of LV weight to body weight(LVW/BW)and the cross-sectional area of cardiomyocytes were measured.The mRNA expression level of ROCK was detected by RT-PCR.The expression level of Phosphorylated-ERM was detected by immunohisto-chemistry and Western blot.Results:28ds after MI

the LVW/BW

the cross-sectional area of cardiomyocytes

the mRNA expression level of ROCK and the expression level of Phosphorylated-ERM significantly increased in the MI group compared with those in the SHAM group.And the indexes mentioned above significantly decreased both in the SIM group and F group compared with those in the MI group.Conclusion:Simvastatin prevents post-infarcted cardiomyocyte hypertrophy partly through inhibition of ROCK.

关键词

心肌梗死心肌细胞肥大辛伐他汀RhoA激酶

Keywords

myocardial infarctionCardiac hypertrophysimvastatinRhoA kinase

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