Effect of GS K-3β on the cell cycle and metastasis of human gastric carcinoma cell

WANG Yan-li; et al

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Effect of GS K-3β on the cell cycle and metastasis of human gastric carcinoma cell

更新时间：2024-01-17

- Effect of GS K-3β on the cell cycle and metastasis of human gastric carcinoma cell
- Journal of Chongqing Medical University Vol. 34, Issue 4, Pages: 405-408(2009)
- 作者机构：
  
  重庆医科大学附属第一医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.2
- Published：2009，
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[1]王艳丽,高青,谢霞.糖原合酶激酶3-β对胃癌细胞生长周期和转移的影响[J].重庆医科大学学报,2009,34(04):405-408.

WANG Yan-li, et al. Effect of GS K-3β on the cell cycle and metastasis of human gastric carcinoma cell. [J]. Journal of Chongqing Medical University 34(4):405-408(2009)
[1]王艳丽,高青,谢霞.糖原合酶激酶3-β对胃癌细胞生长周期和转移的影响[J].重庆医科大学学报,2009,34(04):405-408. DOI：

WANG Yan-li, et al. Effect of GS K-3β on the cell cycle and metastasis of human gastric carcinoma cell. [J]. Journal of Chongqing Medical University 34(4):405-408(2009) DOI：

摘要

目的:探讨GSK-3β功能改变对胃癌细胞生长周期和转移的影响。方法:LY294002单独或联合LiCl影响胃癌细胞株SCG-7901GSK-3β活性

Western blotting检测总GSK-3β、p-GSK-3βser9表达

MTT和FCM检测细胞增殖及周期分布

细胞免疫化学检测E-cadherin、β-catenin表达

细胞粘附实验观察细胞与基质的粘附力。结果:LY294002干预细胞72h后

总GSK-3β表达无改变

p-GSK-3βser9表达下降;增殖受抑制;细胞周期阻滞于G0/G1期;E-cadherin表达增高

β-catenin表达下降;细胞与基质粘附力降低。LiCl部分拮抗LY294002对细胞的生长抑制、周期阻滞及对E-cadherin、β-catenin表达的改变和细胞与基质粘附力的影响。结论:GSK-3β可促进胃癌细胞G1-S期周期阻滞、抑制细胞生长并可上调E-cadherin表达抑制细胞转移。

Abstract

Objective:To observe the effect of GSK-3β on the cell cycle and metastasis of human gastric carcinoma cell.Methods:Influencing the GSK-3β activity of human gastric carcinoma cell line SCG-7901 by LY294002 with or with not LiCl treatment.The expression of total GSK-3β and p-GSK-3βser9 were detected in protein level by western blotting

cell proliferation and cell cycle distribution were tested by MTT and FCM respectively

the expression of E-cadherin and β-catenin were measured with S-P immunohistochemistry and the cell-ground substance adhesion was tested by cell adherence assay.Results:The expression of total GSK-3β was constant and the protein level of p-GSK-3β ser9 was down regulated after exposured to LY294002 by 72 h

the cell proliferation was inhibited

cell cycle was arrest at G0/G1 phase

the E-cadherin level was positively regulated

the expression of β-catenin and the adhesion rate were decreased.The effect on the proliferation、cell cycle、the expression of E-cadherin and β-catenin and the adhesion by the LY294002 were partially abrogated by lithium chloride.Conclusion:GSK-3βcould negatively regulate the proliferation of human gastric carcinoma cell by leading cells to G1-S arrest and inhibit its metastasis by positively regulating the expression of E-cadherin.

关键词

糖原合酶激酶3-β胃癌细胞周期E-cadherin

Keywords

Glycogen synthase kinase-3βgastric carcinomacell cycleE-cadherin

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