Expression of embryonic stem cells key transcription factor Nanog in bladder cancer and its relationship with cancer stem cells

WANG Wei-sheng; et al

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Expression of embryonic stem cells key transcription factor Nanog in bladder cancer and its relationship with cancer stem cells

更新时间：2024-01-17

- Expression of embryonic stem cells key transcription factor Nanog in bladder cancer and its relationship with cancer stem cells
- Journal of Chongqing Medical University Vol. 34, Issue 4, Pages: 409-412(2009)
- 作者机构：
  
  重庆医科大学附属第一医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.14
- Published：2009，
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[1]王卫生,唐伟.胚胎干细胞关键因子Nanog在膀胱癌中的表达及与肿瘤干细胞的关系[J].重庆医科大学学报,2009,34(04):409-412.

WANG Wei-sheng, et al. Expression of embryonic stem cells key transcription factor Nanog in bladder cancer and its relationship with cancer stem cells. [J]. Journal of Chongqing Medical University 34(4):409-412(2009)
[1]王卫生,唐伟.胚胎干细胞关键因子Nanog在膀胱癌中的表达及与肿瘤干细胞的关系[J].重庆医科大学学报,2009,34(04):409-412. DOI：

WANG Wei-sheng, et al. Expression of embryonic stem cells key transcription factor Nanog in bladder cancer and its relationship with cancer stem cells. [J]. Journal of Chongqing Medical University 34(4):409-412(2009) DOI：

摘要

目的:检测胚胎干细胞（Embnyonic stem cell

ES）自我更新的关键调节因子Nanog在人膀胱癌组织中的表达

探讨Nanog的表达在膀胱癌发生发展中的意义及与肿瘤干细胞的关系。方法:采用半定量RT-PCR检察32例膀胱癌Nanog的表达

32例癌旁非癌膀胱组织

用免疫组织化学方法在蛋白质水平检测Nanog的表达。结果:Nanog在膀胱癌中的表达率为84.38%（27/32）

而在非癌膀胱组织低水平（P<0.01）表达（5/32）

免疫组织化学方法分析结果显示:Nanog主要的集中在肿瘤组织的细胞胞核

在非癌组织中没有或低免疫反应性

Nanog表达的阳性细胞率及强度与膀胱癌的分级、复发和转移无明显相关性（P>0.05）。结论:膀胱癌中有少量细胞表达全能干细胞标志物Nanog

这些细胞很可能是膀胱癌干细胞

这将为揭示膀胱肿瘤干细胞的存在并分离和鉴定膀胱肿瘤干细胞提供依据

Nanog在膀胱癌中的表达表明胚胎基因与肿瘤的发生发展关系密切。

Abstract

Objective:To study the expression of nanog in human bladder cancer tissue

and demonstrate its significance in bladder cancer formation and relationship with bladder cancer stem cells.Methods:The expression of nanog was examined in the pathological specimens from 32 cases of bladder cancers

15 apparently nontumor tissues taken from the margin of tumors and 10 normal urothelial tissues by using semiquantitative RT-PCR and immunohistochemistry respectively.Results:The expression rate of nanog in bladder cancers was 84.38%(27/32)but at much lower level(P<0.001)in some nonneoplastic samples(5/32)

According to immunohistochemical (IHC)results

nanog is primarily localized in the nuclei of tumor cells

with no or lowimmunoreactivity in nontumor cells.the positive rate and intensity of the nanog expression had no obvious relationship with the grade

recurrence or metastasis of bladder cancer(P>0.05). Conclusion:The expression of nanog showed there might exist bladder cancer stern cells.This has significant aplications to uncover the existence of CSC and to isolate and identificate bladder cancer stem cells.The rate and level of nanog expression in bladder cancer showed there might exist significant correlation between embryonic gene and occurrence and development of tumor.

关键词

Nanog膀胱肿瘤肿瘤干细胞

Keywords

NanogBladder cancerCancer stem cells

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