Expression and activity detection of IL3-PE38KDEL

SHEN Yan; et al

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Expression and activity detection of IL3-PE38KDEL

更新时间：2024-01-17

- Expression and activity detection of IL3-PE38KDEL
- Journal of Chongqing Medical University Issue 11, Pages: 1362-1364(2008)
- 作者机构：
  
  重庆医科大学附属第二医院血液科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392;R733.71
- Published：2008，
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[1]沈燕,陈妤,娄世锋.免疫毒素IL-3-PE38KDEL的表达及活性测定[J].重庆医科大学学报,2008(11):1362-1364.

SHEN Yan, et al. Expression and activity detection of IL3-PE38KDEL. [J]. Journal of Chongqing Medical University (11):1362-1364(2008)
[1]沈燕,陈妤,娄世锋.免疫毒素IL-3-PE38KDEL的表达及活性测定[J].重庆医科大学学报,2008(11):1362-1364. DOI：

SHEN Yan, et al. Expression and activity detection of IL3-PE38KDEL. [J]. Journal of Chongqing Medical University (11):1362-1364(2008) DOI：

摘要

目的:制备具有杀伤活性的新型抗白血病免疫毒素。方法:在异丙基硫代-β-D-半乳糖苷（Isopropyl-beta-D-throgalac-top yranoside

IPTG）的诱导下

高效表达免疫毒素IL3-PE38KDEL

采用细胞毒性实验

对毒素活性进行检测。结果:免疫毒素IL3-PE38KDEL以包涵体形式为主高效表达

该蛋白具有较好的毒素活性。结论:免疫毒素IL3-PE38KDEL具有良好的生物学活性

为其作为抗白血病药物的进一步研究打下了基础。

Abstract

Objective:To prepare a new type of anti-leukemia immunotoxin with killing activity.Methods:The method of cytotoxicity was used to study the activity of the immunotoxin after the induction of IPTG. Results:The expressed fusion proteins were detected mostly as inclusion bodies at high level.The result showed IL3-PE38KDEL had liable activity of toxicity. Conclusion:The fusion protein IL3-PE38KDEL has good biological activity

which paves way for the further study on its treatment of leukemia.

关键词

白介素3假单胞菌外毒素免疫毒素

Keywords

Human interleukin-3Pseudomonas exotoxin(PE38KDEL)Immunotoxin

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