Study on survivin mRNA and protein of BEP-2D cells during its malignant transformation by cigarette smoke condensate

XIE Jun-hao; et al

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Study on survivin mRNA and protein of BEP-2D cells during its malignant transformation by cigarette smoke condensate

更新时间：2024-01-17

- Study on survivin mRNA and protein of BEP-2D cells during its malignant transformation by cigarette smoke condensate
- Journal of Chongqing Medical University Vol. 34, Issue 1, Pages: 49-51(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院胸心外科
  2. 军事医学科学院放射与辐射医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R363
- Published：2009，
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[1]谢俊豪,吴庆琛,张诚,李强,朱茂祥,杨陟华,潘秀颉.烟草悬凝物诱导永生化人支气管上皮细胞恶性转化过程中survivin基因及蛋白表达的研究[J].重庆医科大学学报,2009,34(01):49-51.

XIE Jun-hao, et al. Study on survivin mRNA and protein of BEP-2D cells during its malignant transformation by cigarette smoke condensate. [J]. Journal of Chongqing Medical University 34(1):49-51(2009)
[1]谢俊豪,吴庆琛,张诚,李强,朱茂祥,杨陟华,潘秀颉.烟草悬凝物诱导永生化人支气管上皮细胞恶性转化过程中survivin基因及蛋白表达的研究[J].重庆医科大学学报,2009,34(01):49-51. DOI：

XIE Jun-hao, et al. Study on survivin mRNA and protein of BEP-2D cells during its malignant transformation by cigarette smoke condensate. [J]. Journal of Chongqing Medical University 34(1):49-51(2009) DOI：

摘要

目的:探讨人支气管上皮细胞（BEP-2D）恶性转化过程中survivin基因及蛋白表达的变化。方法:选取正常BEP-2D细胞及经烟草悬凝物处理后的第15代（P-15）、25代（P-25）、38代（P-38）BEP-2D细胞共4组细胞株作为研究对象

采用半定量反转录PCR（Retro-translation PCR

RT-PCR）检测各组细胞株中survivin基因变化

免疫组织化检测各组中survivin蛋白的表达。结果:1.survivin mRNA在正常细胞BEP-2D中的表达强度为0.08

在转化的肺癌细胞P-15、P-25和P-38中分别为0.56、0.80和0.81。2.survivin蛋白在正常BEP-2D细胞株中表达阴性

在转化的3组细胞中表达阳性

且survivin蛋白在正常细胞与转化细胞株中的表达存在明显差异

其中在P-15具有明显差异（P<0.05）。结论:survivin基因及蛋白的表达水平可作为早期肺癌的一个检测指标。

Abstract

Objective:To study the change of the survivin mRNA and protein of BEP-2D cells during its malignant transformation.Methods:Normal BEP-2D cell and BEP-2D cells treated by cigarette smoke condensate(CSC)for 15 weeks(P-15)

25 weeks(P-25)and 38 weeks(P-38)were respectively chosen to study the survivin gene and protein by RT-PCR(retro-translation PCR

RT-PCR)and immunohistochemical method.Results:The survivin mRNA was found in BEP-2D cells of P-15

P-25 and P-38

respectively with 0.56

0.80

and 0.81

but not in the normal BEP-2D cell.The survivin protein was found in the normal BEP-2D cell

but not in BEP-2D cells of P-15

P-25 and P-38.The levels of survivin protein expressian were different between BEP-2D cells of P-15

P-25 and P-38 and normal BEP-2D cell

with significant difference between P-15 BEP-2D cell and normal BEP-2D cell(P<0.05).Conclusion:The survivin mRNA and protein may be consider of a kind of biology-marker for the early diagnosis of lung cancer because the survivin mRNA and protein occur in the earlier period of lung cancer.

关键词

烟草悬凝物永生化人支气管上皮细胞survivin基因及蛋白免疫组化半定量反转录PCR

Keywords

Cigarette smoke condensate(CSC)Immortalized human bronchial epithelial cells(BEP-2D)survivin mRNA and proteinimmunohistochemicalRetro-translation PCR(RT-PCR)

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