Effects of hehodge signaling pathway on poliferation,apoptosis and cyclinD1 expression of breast cancer cell line MDA-MB-231

HUANG Wei; et al

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Effects of hehodge signaling pathway on poliferation,apoptosis and cyclinD1 expression of breast cancer cell line MDA-MB-231

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- Effects of hehodge signaling pathway on poliferation,apoptosis and cyclinD1 expression of breast cancer cell line MDA-MB-231
- Journal of Chongqing Medical University Vol. 34, Issue 1, Pages: 45-48(2009)
- 作者机构：
  
  重庆医科大学基础医学院病理学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2009，
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[1]黄伟,张徽,姜小良,刘香丽.Hedgehog信号转导途径对乳腺癌MDA-MB-231细胞增殖与凋亡以及对CycinD1表达的影响[J].重庆医科大学学报,2009,34(01):45-48.

HUANG Wei, et al. Effects of hehodge signaling pathway on poliferation,apoptosis and cyclinD1 expression of breast cancer cell line MDA-MB-231. [J]. Journal of Chongqing Medical University 34(1):45-48(2009)
[1]黄伟,张徽,姜小良,刘香丽.Hedgehog信号转导途径对乳腺癌MDA-MB-231细胞增殖与凋亡以及对CycinD1表达的影响[J].重庆医科大学学报,2009,34(01):45-48. DOI：

HUANG Wei, et al. Effects of hehodge signaling pathway on poliferation,apoptosis and cyclinD1 expression of breast cancer cell line MDA-MB-231. [J]. Journal of Chongqing Medical University 34(1):45-48(2009) DOI：

摘要

目的:本实验通过Hedgehog（HH）信号转导途径的抑制剂Cyclopamine作用于人乳腺癌MDA-MB-231细胞

研究Hedgehog信号转导途径对乳腺癌MDA-MB-231细胞增殖与凋亡以及对CycinD1表达的影响。方法:以MDA-MB-231细胞为研究对象

应用Cyclopamine处理后

用MTT方法检测细胞增殖活性的改变;用流式细胞术检测细胞周期与凋亡的情况;RT-PCR检测GLI1、CyclinD1的mRNA的变化;用免疫细胞化学术检测细胞中的CyclinD1蛋白表达的变化。结果:与对照组相比

Cyclopamine可显著抑制MDA-MB-231细胞的生长并呈时效-量效关系;流式细胞术检测MDA-MB-231细胞

发现Cyclopamine能提高细胞周期G0/G1期的比例

48h后出现明显的"亚G1"峰;GLI1

CyclinD1的mRNA以及CyclinD1蛋白的表达降低。结论:乳腺癌MDA-MB-231细胞有Hedgehog信号转导途径的激活;Hedgehog信号转导途径的抑制剂Cyclopamine能明显抑制MDA-MB-231细胞生长并能诱导其凋亡

减少CyclinD1的表达。

Abstract

Objective:To research the effects of HH singaling pathway on proliferation

apoptosis and CyclinD1 expression of breast cancer MDA-MB-231 cells by MDA-MB-231 cells treated with Cyclopamine

a HH singaling pathway inhibitor.Methods:MDA-MB-231 cells were treated with Cyclopamine.Cell proliferation was detected by MTT;Cell cycle distribution and apoptosis were analysed by flow cytometry(FCM).The mRNA levels of GLI1

CyclinD1 were measured by reverse transcription-polymerase chain reaction(RT-PCR);The protein level of CyclinD1 was detected by immunostaining of cell lines.Results:Compared with control group

Cyclopamine could significantly inhibit the proliferation of MDA-MB-231 cells in a dose-and time-dependent manner;By FCM the treatment of MDA-MB-231 cells with Cyclopamine induced a remarkable increase in the proportion of cells in the G0/G1 phase of the cell cycle and after 48 h the sub-G1 peak appeared.The mRNA levels of GLI1

CyclinD1 and the expression of CyclinD1 protein were reduced.Conclusion:The HH signaling pathway was activeted in MDA-MB-231 cells.HH signaling pathway inhibitor

Cyclopamine

could inhibit the proliferation

induce the apoptosis and reduce the expression of CyclinD1 in MDA-MB-231 cells.

关键词

HedgehogGLI1cyclinD1信号转导Cyclopamine

Keywords

hedgehogGLI1cyclinD1Signal transductionCyclopamine

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