In vitro MR imaging of superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells from SD rat

HANG You-xin; et al

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In vitro MR imaging of superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells from SD rat

更新时间：2024-01-17

- In vitro MR imaging of superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells from SD rat
- Journal of Chongqing Medical University Vol. 34, Issue 3, Pages: 318-321(2009)
- 作者机构：
  
  重庆医科大学附属第一医院放射科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.1
- Published：2009，
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[1]黄铀新,吕富荣,吕发金,肖云华,马秀华,肖智博.超顺磁性氧化铁标记大鼠骨髓间充质干细胞的磁共振成像研究[J].重庆医科大学学报,2009,34(03):318-321.

HANG You-xin, et al. In vitro MR imaging of superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells from SD rat. [J]. Journal of Chongqing Medical University 34(3):318-321(2009)
[1]黄铀新,吕富荣,吕发金,肖云华,马秀华,肖智博.超顺磁性氧化铁标记大鼠骨髓间充质干细胞的磁共振成像研究[J].重庆医科大学学报,2009,34(03):318-321. DOI：

HANG You-xin, et al. In vitro MR imaging of superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells from SD rat. [J]. Journal of Chongqing Medical University 34(3):318-321(2009) DOI：

摘要

目的:探讨超顺磁性氧化铁纳米粒子（Superparamagnetic iron oxide

SPIO）体外标记大鼠骨髓间充质干细胞（Bone marrow mesenchymal stem cells

BMSCs）的适当浓度和不同标记浓度对细胞的生物学活性影响

以及经MR成像的特征等。方法:选取第5代细胞进行不同浓度SPIO标记

运用光学显微镜观察铁颗粒在细胞内的位置、分布及标记率;选取适当浓度标记量

使用1.5T磁共振进行T1WI、T2WI、T2*WI扫描

测量不同扫描序列标记细胞管的信号强度改变

并进行统计学分析。结果:标记后的铁颗粒均位于细胞质内;含量在25～50μg Fe/ml的培养液是SPIO标记干细胞的安全浓度

在此浓度阈值标记后孵育24h即可有效标记细胞97%

00%;SPIO标记的MSCs在T1WI

T2WI及T2*WI序列信号均降低。结论:SPIO可以简便标记MSCs。并且在适当浓度下对MSCs的生物学活性没有影响

MR T2WI和T2*WI序列可有效显像磁性标记的干细胞

为下一步活体试验奠定基础。

Abstract

Objective:To explore the proper concentration of superparamagnetic iron oxide (SPIO) labeling bone marrow mesechymal stem cells and the biological influence of different labeling concentrations

and to evaluate the characteristics of magnetic resonance imaging by a 1.5 T MR scanner. Methods:BMSCs (5th)were incubated with different concentrations of SPIO particles. The location

distribution and the labeling ratio of SPIO particles in cells were observed. At an optimal concentration

samples of SPIO-labeled and unlabeled MSCs suspension in agar were imaged by MRI with T1WI

T2WI and T2*WI. and the signal intensity were measured and statistically analyzed.Results:Labeled SPIO particles were stained in cytoplasm. BMSCs were efficiently labeled (97% to 100%)in culture at 24 hours and particle concentrations(25 to 50μg Fe/ml medium). Compared with unlabeled cells

BMSCs loaded with SPIO had similar viabilitv and proliferation profiles at this proper range of labeling concentration. SPIO labeling caused a stronger low signal attenuation effect in T1WI、T2WI andT2*WI. Conclusion:BMSCs can be easily and efficiently labeled by SPIO without interference on the cell viability and proliferation

MRI visualization of SPIO-labeled MSCs is feasible

which provrdes basis for future in vivo studies.

关键词

干细胞超顺磁性氧化铁磁共振成像

Keywords

StemcellsSuperparamagneticironoxidmagneticresonanceimaging

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