Changes in caspase-3,8 activity and mRNA expression of normal human hepato-cyte HL-7702 cell in the process of apoptosis induced by glycodeoxycholate

CHUAN Liang-min; et al

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Changes in caspase-3,8 activity and mRNA expression of normal human hepato-cyte HL-7702 cell in the process of apoptosis induced by glycodeoxycholate

更新时间：2024-01-17

- Changes in caspase-3,8 activity and mRNA expression of normal human hepato-cyte HL-7702 cell in the process of apoptosis induced by glycodeoxycholate
- Journal of Chongqing Medical University Vol. 34, Issue 10, Pages: 1338-1340(2009)
- 作者机构：
  
  四川省医学科学院·四川省人民医院检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R575
- Published：2009，
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[1]传良敏,黄文芳,饶绍琴,洪华,邓君,杨永长.甘氨脱氧胆酸盐诱导人正常肝细胞株HL-7702凋亡过程中caspase-3,8活性与mRNA表达水平变化[J].重庆医科大学学报,2009,34(10):1338-1340.

CHUAN Liang-min, et al. Changes in caspase-3,8 activity and mRNA expression of normal human hepato-cyte HL-7702 cell in the process of apoptosis induced by glycodeoxycholate. [J]. Journal of Chongqing Medical University 34(10):1338-1340(2009)
[1]传良敏,黄文芳,饶绍琴,洪华,邓君,杨永长.甘氨脱氧胆酸盐诱导人正常肝细胞株HL-7702凋亡过程中caspase-3,8活性与mRNA表达水平变化[J].重庆医科大学学报,2009,34(10):1338-1340. DOI：

CHUAN Liang-min, et al. Changes in caspase-3,8 activity and mRNA expression of normal human hepato-cyte HL-7702 cell in the process of apoptosis induced by glycodeoxycholate. [J]. Journal of Chongqing Medical University 34(10):1338-1340(2009) DOI：

摘要

目的:观察甘氨脱氧胆酸盐（Glycodeoxycholate

GCDC）诱导人正常肝细胞株HL-7702凋亡过程中caspase-3

8活性与mRNA表达水平的变化

探讨GCDC诱导肝细胞凋亡的机制。方法:体外培养HL-7702细胞

不同浓度的GCDC为处理因素

用AnnexinⅤ-FITC/PI双染色法结合流式细胞技术仪检测细胞凋亡率

比色法测定caspase-3

8活性

RT-PCR检测caspase-3

8mRNA表达水平。结果:100～250μmol/L的GCDC处理HL-7702细胞24 h后

其细胞凋亡率、caspase-3

8活性与mRNA表达水平明显升高

并与GCDC呈浓度依赖性。结论:caspase-3

8参与GCDC诱导HL-7702细胞凋亡的调控

GCDC通过活化caspase-8

并进一步活化caspase-3从而诱导肝细胞凋亡。

Abstract

Objective:To investigate the apoptosis and changes of caspase-3

8 activity and mRNA expression induced by glycodeoxycholate(GCDC) in cultured normal human hepatocyte HL-7702 cell in vitro and to explore the possible mechanism of hepatocyte apoptosis.Methods:HL-7702 cells were cultured with GCDC

the final concentration of which were 100、150、200 and 250 μmol/L respectively.The apoptosis ratio of HL-7702 cell was determined by AnnexinⅤ-FITC/PI double staining.Colorimetric method was used to measure caspase-3

8 activity.The expression of caspase-3

8 mRNA was determined by RT-PCR.Results:When hepatocytes were incubated with GCDC for 24 hours

the apoptotic ratio of hepatocyte

caspase-3

8 activity and mRNA expression of HL-7702 cells increased remarkably and were concentration-dependent on GCDC.Conclusion: The caspase-3

8 were concerned with the hepatocyte apoptosis induced by GCDC.The mechanism might be related to the upregulation of caspase-8 activity which subsequently transforms caspase-3 into its active form.

关键词

甘氨脱氧胆酸盐细胞凋亡Caspase

Keywords

GlycodeoxycholateapoptosisCaspase

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