Empirical study on ursolic acid inducing apoptosis in human prostatic carcinoma cell line PC-3

XIA Yang; et al

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Empirical study on ursolic acid inducing apoptosis in human prostatic carcinoma cell line PC-3

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- Empirical study on ursolic acid inducing apoptosis in human prostatic carcinoma cell line PC-3
- Journal of Chongqing Medical University Vol. 34, Issue 12, Pages: 1661-1665(2009)
- 作者机构：
  
  重庆医科大学附属第一医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.25
- Published：2009，
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[1]夏阳,苟欣.熊果酸诱导前列腺癌细胞株PC-3凋亡的实验研究[J].重庆医科大学学报,2009,34(12):1661-1665.

XIA Yang, et al. Empirical study on ursolic acid inducing apoptosis in human prostatic carcinoma cell line PC-3. [J]. Journal of Chongqing Medical University 34(12):1661-1665(2009)
[1]夏阳,苟欣.熊果酸诱导前列腺癌细胞株PC-3凋亡的实验研究[J].重庆医科大学学报,2009,34(12):1661-1665. DOI：

XIA Yang, et al. Empirical study on ursolic acid inducing apoptosis in human prostatic carcinoma cell line PC-3. [J]. Journal of Chongqing Medical University 34(12):1661-1665(2009) DOI：

摘要

目的:探讨熊果酸（Ursolic acid

UA）对人前列腺癌细胞株PC-3细胞周期、凋亡的影响及其作用机制。方法:人前列腺癌细胞株PC-3以不同浓度的UA作用不同的时间后

分别使用四甲基偶氮唑蓝比色法（4 methyl thiazolyl tetrazolium assay

MTT）

免疫组织化学SP法

流式细胞术（Flow cytometry

FCM）

Western blot等实验方法检测PC-3细胞的增殖抑制情况

细胞周期和凋亡率的变化

以及细胞内bcl-2、bax2个重要的细胞凋亡相关基因和细胞凋亡相关特殊蛋白cox-2、caspase3表达的变化。结果:UA对PC-3细胞有增殖抑制作用

其作用随着药物浓度和作用时间的增加而增强。24、48h和72h3个时间段UA分别作用于PC-3细胞后的IC50分别为50.87、37.91μmol/L和27.86μmol/L;熊果酸作用后PC-3细胞周期的主要特点是大量细胞累积于G1期

进入S期的细胞减少。UA能够诱导PC-3细胞凋亡

经过24、48h和72h3个时间段UA浓度50μmol/L作用后

细胞凋亡率为6.21%、7.81%和13.31%;细胞内bcl-2和cox-2的表达随着药物浓度增加逐渐减少;bax和caspase3蛋白的表达随着药物浓度增加逐渐增加。结论:UA能够抑制PC-3细胞的增殖

并使细胞大量累积于G1期

进入S期的细胞减少。可能通过使bcl-2和cox-2的表达减少、bax和caspase3蛋白的表达增加来诱导细胞凋亡。

Abstract

Objective:To investigate the proliferation inhibition and apoptosis in human prostatic carcinoma cell line PC-3 induced by ursolic acid and their mechanism.Methods:PC-3 were treated by ursolic acid with different concentration and time.MTT assay

flow cytometry(FCM)

SP method of immunocytochemistry

Western blot were used to observe the cell proliferation inhibition

cell cycle and apoptosisrate

the expressions of bcl-2

cox-2

bax and caspase 3.Results:Ursolic acidinhibited the proliferation of PC-3 cells in dosage and time dependent manner.The IC50 of PC-3 cells that were treated by ursolic acid for 24

48 h and 72 h are 50.87

37.91μmol/L and 27.86 μmol/L.The apoptosis rate of the PC-3 cells hightened with the increasing of the ursolic acid density and time.The apoptosis rates of the PC-3 cells that treated by 50 μmol/L ursolic acid for 24

48 h and 72 h are 6.21

7.81% and 13.31%.Ursolic acid induced a G1 cell cycle arrest and reduced the cell population in S phase.With the increasing of the medicine density

the expression of bcl-2 and cox-2 was down-regulated

while the expression of bax and caspase 3 up-regulated in the apoptosis.Conclusion:Ursolic acid can inhibit proliferation of the PC-3 cells and induced a G1 cell cycle arrest and reduced the cellpopulation in S phase.It also can induce apoptosis of human prostatic carcinoma through down-regulating the expressions of bcl-2 and cox-2

up-regulating the expression of bax

and caspase3.

关键词

熊果酸前列腺癌PC-3细胞凋亡

Keywords

ursolic acidProstatic carcinoma cell line PC-3apoptosis

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