Prevalence and molecular mechanism of helicobacter pylori resistant to clarithromycin in Chongqing area

LUO Hong-chun; et al

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Prevalence and molecular mechanism of helicobacter pylori resistant to clarithromycin in Chongqing area

更新时间：2024-01-17

- Prevalence and molecular mechanism of helicobacter pylori resistant to clarithromycin in Chongqing area
- Journal of Chongqing Medical University Vol. 34, Issue 8, Pages: 1077-1080(2009)
- 作者机构：
  
  重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R446.5
- Published：2009，
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[1]罗红春,吕琳,杨致邦,梅浙川.重庆地区分离幽门螺杆菌对克拉霉素耐药性研究[J].重庆医科大学学报,2009,34(08):1077-1080.

LUO Hong-chun, et al. Prevalence and molecular mechanism of helicobacter pylori resistant to clarithromycin in Chongqing area. [J]. Journal of Chongqing Medical University 34(8):1077-1080(2009)
[1]罗红春,吕琳,杨致邦,梅浙川.重庆地区分离幽门螺杆菌对克拉霉素耐药性研究[J].重庆医科大学学报,2009,34(08):1077-1080. DOI：

LUO Hong-chun, et al. Prevalence and molecular mechanism of helicobacter pylori resistant to clarithromycin in Chongqing area. [J]. Journal of Chongqing Medical University 34(8):1077-1080(2009) DOI：

摘要

目的:了解重庆地区幽门螺杆菌（Heliobacter pylori

Hp）对克拉霉素的耐药情况

探讨Hp对克拉霉素耐药的分子机制。方法:分离培养获得96株Hp。采用琼脂稀释法进行体外抗生素敏感试验

检测Hp对克拉霉素的最低抑菌浓度（Minimal inhibitory concentration

MIC）

拟定克拉霉素MIC>2μg/ml为耐药菌株。比较城市和农村的差异。挑选克拉霉素耐药Hp10株

提取细菌DNA

用PCR-限制性片段长度多态性（RFLP）分析探讨克拉霉素耐药机制。结果:重庆地区Hp对克拉霉素的耐药率为29.17%

城市和农村耐药率无统计学差异（P>0.05）。10株克拉霉素耐药菌株23SrRNA基因功能区VPCR扩增片段

均被BsaⅠ酶切

未被BbsⅠ酶切

提示10株在2143位点有A→G突变。结论:重庆地区Hp对克拉霉素的耐药率较高

且城市和农村无显著差异。大多数克拉霉素耐药Hp存在23SrRNA基因功能区V2143位点A→G突变。

Abstract

Objective:To investigate the prevalence and resistance mechanism of Helicobacter pylori(Hp) resistant to Clarithromycin in Chongqing area.Methods:96 Hp clinical isolates were cultured.The susceptibilities of the 96 Hp strains to clarithromycin were tested by agar dilution test to determine minimum inhibitory concentration(MIC).These Hp strains with MIC>2 μg/ml were defined to be resistant to clarithromycin

then prevalence of Hp resistant to Clarithromycin in urban and rural area of Chongqing were compared.10 Hp strains of being resistant to Clarithromycin were chosen.After the DNAs of Hp were extracted

PCR-Restriction Fragment Length Polymorphism(RFLP) analysis was used to test 23SrRNA domain V gene mutations.Results:The resistance rate of Hp resistant to Clarithromycin in Chongqing area was 29.17%

with no statistically significant difference between urban and rural area(P>0.05).10 clarithromycin-resistant stains of Hp were found to have an A-to-G mutation in 23SrRNA domain V at position of 2143.Conclusions:The results indicated that prevalence of Hp resistant to Clarithromycin in Chongqing area was higher

and there was no statistically significant difference between urban and rural area(P>0.05).Besides

the majority of clarithromycin-resistant isolates of Hp in Chongqing have an A2143G mutation in 23SrRNA domain V.

关键词

幽门螺杆菌克拉霉素耐药性突变

Keywords

Helicobacter pyloriClarithromycinresistancemutation

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