Effect of intracellular nitric oxide on the apoptosis of K562 cell induced by simvastatin

ZHANG Juan; et al

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Effect of intracellular nitric oxide on the apoptosis of K562 cell induced by simvastatin

更新时间：2024-01-17

- Effect of intracellular nitric oxide on the apoptosis of K562 cell induced by simvastatin
- Journal of Chongqing Medical University Vol. 34, Issue 10, Pages: 1317-1320(2009)
- 作者机构：
  
  1. 重庆医科大学医学检验系
  2. 四川绵阳四零四医院检验科
  3. 四川省医学科学院四川省人民医院检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R733.7
- Published：2009，
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[1]张娟,曾娅莉,黄文芳,刘华,杨永长,刘俭,何静.一氧化氮信号在辛伐他汀诱导K562细胞凋亡过程的作用[J].重庆医科大学学报,2009,34(10):1317-1320.

ZHANG Juan, et al. Effect of intracellular nitric oxide on the apoptosis of K562 cell induced by simvastatin. [J]. Journal of Chongqing Medical University 34(10):1317-1320(2009)
[1]张娟,曾娅莉,黄文芳,刘华,杨永长,刘俭,何静.一氧化氮信号在辛伐他汀诱导K562细胞凋亡过程的作用[J].重庆医科大学学报,2009,34(10):1317-1320. DOI：

ZHANG Juan, et al. Effect of intracellular nitric oxide on the apoptosis of K562 cell induced by simvastatin. [J]. Journal of Chongqing Medical University 34(10):1317-1320(2009) DOI：

摘要

目的:观察一氧化氮（Nitricoxide

NO）信号在辛伐他汀（Simvastain）诱导K562细胞凋亡过程中变化

推测一氧化氮信号与K562细胞凋亡之间关系。方法:20μmol/L辛伐他汀处理K562细胞24、48和72 h;流式细胞技术检测细胞凋亡率;比色法测定K562细胞内总一氧化氮浓度;RT-PCR检测诱导型一氧化氮合成酶（Inducible nitric oxide synthase

iNOS）基因;一氧化氮抑制剂和20μmol/L辛伐他汀共同培养

观察不同时间细胞凋亡率。结果:与对照组比较

24、48 h和72 h处理组细胞凋亡率增加6.1%±0.4%

14.2%±0.4%

30.7%±0.6%

差异具有显著性;不同时间处理诱导性一氧化氮基因均显著上调;处理组细胞内总一氧化氮浓度增加为（0.3±0.1）mg/ml

（2.8±0.2）mg/ml

（3.8±0.4）mg/ml

与对照组比较显著升高;一氧化氮抑制剂能显著降低处理组细胞凋亡率。结论:辛伐他汀能诱导K562细胞凋亡

细胞内升高的一氧化氮浓度可能是辛伐他汀诱导K562细胞凋亡的信号机制之一。

Abstract

Objective:To study the variation of nitric oxide and investigate its relationship with apoptosis in apoptotic K562 cells induced by simvastatin.Methods: K562 cells were exposed to 20 μmol/L concentrations of simvastatin at different times(24

48 and 72 h).Flow cytometry were performed to confirm cell apoptotic ratio.Colorimetric method was used to measure level of intracellular nitric oxide.Reverse transcription-polymerase chain reaction was used to detect the quantity of iNOS mRNA.The apotontic ratio of K562 cells treated with nitric oxide inhibitor and simvastatin were detected at different times.Results: K562 cells could undergo apoptosis after the treatment of 20μmol/L simvastatin for 24

48 and 72 h

and the apoptotic ratios were 6.1%±0.4%

14.2%±0.4%

30.7%±0.6%

respectively.Furthermore

the intracellular nitric oxide levels and iNOS mRNA of K562 cells in treated group were increased remarkably in contrast to the control group.The intracellular nitric oxide levels in the K562 cells of treated group were(0.3±0.1) mg/ml

(2.8±0.2) mg/ml

(3.8±0.4) mg/ml.The cell apoptotic ratios of treated group decreased by addition of nitric oxide inhibitor

as compared with the control group.Conclusion: K562 cells could be induced to apoptosis by simvastatin

the underlying mechanism of which might be related to regulation of increased intracellular nitric oxide levels.

关键词

辛伐他汀一氧化氮细胞凋亡

Keywords

simvastatinnitric oxideapoptosis

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