Simvastatin induces K562 cell apoptosis by oxidative stress

HUANG Wen-fang; et al

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Simvastatin induces K562 cell apoptosis by oxidative stress

更新时间：2024-01-17

- Simvastatin induces K562 cell apoptosis by oxidative stress
- Journal of Chongqing Medical University Vol. 34, Issue 7, Pages: 892-895(2009)
- 作者机构：
  
  1. 四川省医学科学院·四川省人民医院检验科
  2. 绵阳市第四人民医院检验科
  3. 重庆医科大学医学检验系
- 作者简介：
- 基金信息：
- DOI：
  CLC： R733.7
- Published：2009，
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[1]黄文芳,杨永长,曾娅莉,刘华,肖代雯,胥国强,刘文.辛伐他汀通过氧化应激诱导K562细胞凋亡[J].重庆医科大学学报,2009,34(07):892-895.

HUANG Wen-fang, et al. Simvastatin induces K562 cell apoptosis by oxidative stress. [J]. Journal of Chongqing Medical University 34(7):892-895(2009)
[1]黄文芳,杨永长,曾娅莉,刘华,肖代雯,胥国强,刘文.辛伐他汀通过氧化应激诱导K562细胞凋亡[J].重庆医科大学学报,2009,34(07):892-895. DOI：

HUANG Wen-fang, et al. Simvastatin induces K562 cell apoptosis by oxidative stress. [J]. Journal of Chongqing Medical University 34(7):892-895(2009) DOI：

摘要

目的:观察辛伐他汀对K562细胞凋亡的影响

探讨辛伐他汀诱导K562细胞凋亡的分子机制。方法:不同浓度辛伐他汀处理K562细胞

AnnexinⅤ-FITC/PI双染法检测辛伐他汀对K562细胞凋亡的影响。辛伐他汀处理K562细胞48h

流式细胞仪检测活性氧（Reactive oxygen species

ROS）浓度

免疫组织化学法测定突变P53蛋白表达。RT-PCR测定c-junmRNA表达。结果:AnnexinⅤ-FITC/PI双染法检测结果显示

5、10、20μmol/L辛伐他汀作用K562细胞48

2h能诱导其凋亡。辛伐他汀作用K562细胞后

与对照组比较

处理组细胞内ROS明显升高。辛伐他汀处理K562细胞后突变P53蛋白表达明显下调

c-jun mRNA表达上调。结论:辛伐他汀改变K562细胞内氧化还原状态

细胞氧化应激

下调突变P53蛋白表达

上调c-jun表达诱导K562细胞凋亡。

Abstract

Objective: To investigate the effects of simvastatin on K562 cells apoptosis and elucidate its molecular mechanisms. Methods: K562 cells were treated with various concentrations of simvastatin for different time

and then AnnexinⅤ-FITC/PI staining was performed to confirm the apoptosis of K562 cells. Flow cytometry was employed to measure ROS level;mutant P53 protein expression level in K562 cells was measured by immunohistochemistry method. RT-PCR was employed to analyze the mRNA expression levels of c-jun. Results: K562 cells were induced to undergo apoptosis after 5

10 μmol/L and 20 μmol/L simvastatin treatment for 48 h and 72 h. After the treatment of 10 μmol/L and 20 μmol/L simvastatin for 48 h

compared with the control group

ROS level in K562 cells increased obviously. Immunohistochemistry results indicated that simvastatin could inhibit protein expression levels of mutant-type P53. RT-PCR results showed that simvastatin could increase the mRNA expression levels of c-jun significantly. Conclusion: Simvastatin induces K562 cells apoptosis by down-regulating mutant P53 protein and up-regulating c-jun mRNA expression which might be caused by changes of intracellular redox state.

关键词

辛伐他汀氧化应激细胞凋亡

Keywords

simvastatinoxidative stressapoptosis

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