Objective:To explore the effect of gene hepaCAM transfection on the proliferation in human bladder carcinoma cell line. Methods: Plasmid pEGFP-N2-hepaCAM was transfected into T24 cells by Lipofectamine2000

and the T24 cell stably expressing hepaCAM gene was established by G4l8 selection.The expression of hepaCAM mRNA and protein was detected by RT-PCR and Western blot assays. The effects of hepaCAM on cell proliferation were measured by MTT and colony formation assays. The cell cycle progression were measured by flow cytometry. The expression of CyclinD1 mRNA was detected by RT-PCR. Results:Expression of hepaCAM gene and protein were proved by RT-PCR and Western blot assays. The MTT assay showed cell growth in the T24/hepaCAM-GFP group was significantly slow when compared with non-transfection and mock-vehicle groups whereas the rates of clonal formation were greatly decreased(P<0.01). Flow cytometric data showed that more positive clone cells were blocked into G0/G1 phase and fewer cells went into phase S(P<0.05). The expression of CyclinD1 mRNA was lower than non-transfection and mock-vehicle groups. Conclusion: HepaCAM gene might suppress the proliferation of T24 cells through arresting cell cycle in the G0/G1 phase and inhibitting the expression of CyclinD1 mRNA.