The test of fas ligand gene transfection on inducing renal transplant immunotolerance in rats

XU Guang-yong; et al

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The test of fas ligand gene transfection on inducing renal transplant immunotolerance in rats

更新时间：2024-01-17

- The test of fas ligand gene transfection on inducing renal transplant immunotolerance in rats
- Journal of Chongqing Medical University Vol. 34, Issue 9, Pages: 1162-1165(2009)
- 作者机构：
  
  1. 重庆医科大学附属第二医院泌尿外科
  2. 重庆医科大学附属第二医院肝胆外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.4
- Published：2009，
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[1]徐光勇,张唯力,张荣贵,于圣杰,龚建平.FasL基因转染诱导大鼠肾移植免疫耐受的实验研究[J].重庆医科大学学报,2009,34(09):1162-1165.

XU Guang-yong, et al. The test of fas ligand gene transfection on inducing renal transplant immunotolerance in rats. [J]. Journal of Chongqing Medical University 34(9):1162-1165(2009)
[1]徐光勇,张唯力,张荣贵,于圣杰,龚建平.FasL基因转染诱导大鼠肾移植免疫耐受的实验研究[J].重庆医科大学学报,2009,34(09):1162-1165. DOI：

XU Guang-yong, et al. The test of fas ligand gene transfection on inducing renal transplant immunotolerance in rats. [J]. Journal of Chongqing Medical University 34(9):1162-1165(2009) DOI：

摘要

目的:探讨Fas配体（Fasligand

FasL）在大鼠肾移植免疫耐受中的作用及意义。方法:首先建立大鼠同种肾移植模型。实验组供肾移植前用1ml含重组腺病毒Ad-FasL的肾脏冷保存液经肾动脉灌注;建立同种大鼠肾移植模型。分别观察电镜、免疫组织化学法、肾功能测定、生存期

比较各组间的不同。结果:Ad-FasL治疗组肾移植受体大鼠平均存活天数为31.3d

与对照组的平均9.1d存活时间比较

平均存活天数增加了22d

差异显著。Ad-FasL治疗组的移植肾功能基本保持稳定

对照组在术后5d切除对侧肾脏后血清肌酐浓度迅速上升。结论:腺病毒载体可成功介导FasL基因对大鼠肾脏的转染

并对移植肾起免疫保护作用、延长移植肾的存活时间。

Abstract

Objective:To investigate the effect of Fas ligand(FasL) gene transfection to renal allograft in rats. Methods: Firstly Establish renal transplantation model of rat. Secondly FasL recombinant adenovirus vector was contruccted and transduced into rat renal allografts by renal artery perfusion.Immunohistochemistry were used to detect the expression of exogenous FasI gene. Electronic microscopy was used to observe the changes in the ultrastructure.At the same time

mean survival of animals and the level of serum creatinine were observed.Results:Through animal experiment

we found: the mean survival time was 31.3 days in Ad-FasL treated group

whereas the the mean survival time of without treatment group was 9.1 days. The mean survival time increased 22 days in Ad-FasL treated group.There has significant difference. Conclusions:Adenoviral vector can successfully transfection rat kidneys with the FasL cDNA. FasL gene transfection can protect rat renal allografts from immunologic attack and prolong the allograft surviva1.

关键词

肾移植基因转染免疫耐受大鼠

Keywords

kidney transplantationgene transfectionImmunotolerancerat

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