Flow injection chemiluminescence determination of uric acid with micropipe-chip

YI Gang; et al

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Flow injection chemiluminescence determination of uric acid with micropipe-chip

更新时间：2024-01-17

- Flow injection chemiluminescence determination of uric acid with micropipe-chip
- Journal of Chongqing Medical University Vol. 34, Issue 8, Pages: 1063-1065(2009)
- 作者机构：
  
  重庆医科大学检验系、临床检验诊断学省部共建教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R446.1
- Published：2009，
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[1]易钢,钟梁.微通道化学芯片流动注射化学发光测定尿酸[J].重庆医科大学学报,2009,34(08):1063-1065.

YI Gang, et al. Flow injection chemiluminescence determination of uric acid with micropipe-chip. [J]. Journal of Chongqing Medical University 34(8):1063-1065(2009)
[1]易钢,钟梁.微通道化学芯片流动注射化学发光测定尿酸[J].重庆医科大学学报,2009,34(08):1063-1065. DOI：

YI Gang, et al. Flow injection chemiluminescence determination of uric acid with micropipe-chip. [J]. Journal of Chongqing Medical University 34(8):1063-1065(2009) DOI：

摘要

目的:寻求在微通道化学芯片上采用流动注射化学发光分析法快速测定临床样品中尿酸含量。方法:自制的微通道化学芯片

在选定实验条件下

利用尿酸对亚铁氰化钾-铁氰化钾-鲁米诺化学发光体系的增强作用实现血清中尿酸的分析。结果:尿酸浓度在2.0×10-5～1.0×10-2g/L范围内与发光强度增加值线性关系良好;检测限（3σ）为4.6×10-7g/L;精密度试验的RSD为3.09%;加标回收率分别为94.0%、99.7%、107.8%。结论:微通道化学芯片制作方法简单

对血清尿酸含量的流动注射测定灵敏度高

简便快速。

Abstract

Objective:To establish a flow injection chemiluminescence determination method with micropipe-chip for speedy determination of uric acid in clinical samples.Methods:Basing on the rule that uric acid can greatly enhance the chemiluminescence emission intensity of K4Fe(CN)6-K3Fe(CN)6-luminol system

the prepared micropipe-chip was used to analyze the uric acid in serum under the selective conditions.Results:The enhancing chemiluminescence intensity was proportional to uric acid concentration over a range 2.0×10-5～1.0×10-2 g/L.The detection limit(3σ) was 4.6×10-7 g/L.The relative standard deviation(RSD) was 3.09% and the recovery test results were 94.0%

99.7%

and 107.8%.Conclusion:The manufacture technique of micropipr-chip is simple but the proposed determination method of uric acid in human serum is sensitive

fast and convenient.

关键词

微通道化学芯片流动注射化学发光尿酸

Keywords

Micropipe-chipFlow injectionChemiluminescenceuric acid

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Related Institution

Department of Infectious Diseases,Mengchao Hepatobiliary Hospital of Fujian Medical University

Department of Medical Laboratory,Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University

重庆医科大学医学检验系重庆医科大学临床检验诊断学实验室

Department of Cardiology,the Second Affiliated Hospital,Chongqing Medical University

重庆医科大学附属第二医院心血管内科

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