Study on expression and postmortem stability of BDNF after traumatic brain injury in rat

WANG Min; et al

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Study on expression and postmortem stability of BDNF after traumatic brain injury in rat

更新时间：2024-01-17

- Study on expression and postmortem stability of BDNF after traumatic brain injury in rat
- Journal of Chongqing Medical University Vol. 34, Issue 11, Pages: 1521-1523(2009)
- 作者机构：
  
  重庆医科大学基础医学院法医学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R651.15;D919.1
- Published：2009，
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[1]王敏,邓世雄.大鼠脑外伤后BDNF的表达及其死后稳定性的研究[J].重庆医科大学学报,2009,34(11):1521-1523.

WANG Min, et al. Study on expression and postmortem stability of BDNF after traumatic brain injury in rat. [J]. Journal of Chongqing Medical University 34(11):1521-1523(2009)
[1]王敏,邓世雄.大鼠脑外伤后BDNF的表达及其死后稳定性的研究[J].重庆医科大学学报,2009,34(11):1521-1523. DOI：

WANG Min, et al. Study on expression and postmortem stability of BDNF after traumatic brain injury in rat. [J]. Journal of Chongqing Medical University 34(11):1521-1523(2009) DOI：

摘要

目的:探讨脑外伤后脑源性神经营养因子（Brain derived neurotrophical factor

BDNF）表达的变化和损伤时间的关系及其死后稳定性。方法:将健康SD大鼠105只

随机分成4组:正常组、假手术组、脑外伤组、死后稳定性组。采用重物砸伤脑组织建立脑外伤模型（创伤组、稳定性组）

运用免疫组化和图像分析技术

检测各组BDNF的表达变化规律。结果:与正常组、假手术组比较

损伤后0.5 h

BDNF的免疫反应阳性细胞在损伤区周围增多

损伤后6 h BDNF阳性细胞数达高峰

以后逐渐下降

24 h后降至接近正常;死后稳定性组各时间点BDNF表达无显著性差异（P>0.05）。结论:脑外伤后BDNF表达与损伤时间具有相关性;在-20℃条件下

死后稳定性好。

Abstract

Objective:To investigate the expression of BDNF after traumatic brain injury and postmortem stability Methods:105 healthy SD rats were randomly divided into 4 groups:normal group

sham operation group

traumatic brain injury group and postmortem stability group.The model of brain injury were established by weight-drop impact(traumatic brain injury group and postmortem stability group).After that

the regulation of BDNF expression in each group was detected by immunohistochemistry technique

and evaluated with image analysis technique and statistic system.Results:0.5 hour after impact

the BDNF positive neurons increased in each traumatic brain injury group compared with normal group and sham group.6 hour after injury

The BDNF positive neurons reached the peak number.Then

the BDNF positive cells reduced to normal after 24 hours.but there was no significant difference in BDNF expression among each subgroups of postmortem stability group(P>0.05).Conclusion:There is dependability between the BDNF expression after traumatic brain injury and brain injury time

and postmortem stability is nice at-20℃.

关键词

法医病理学免疫组织化学脑外伤脑源性神经营养因子死后稳定性

Keywords

forensic pathologyImmunohisto chemistryBrain injuryBrain derived neurotrophical factor(BDNF)Postmortem stability

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