Effect of Danshengsu on ERK1/2 signal transduction pathway in hepatic stellate cell

LI Zhi; et al

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Effect of Danshengsu on ERK1/2 signal transduction pathway in hepatic stellate cell

更新时间：2024-01-17

- Effect of Danshengsu on ERK1/2 signal transduction pathway in hepatic stellate cell
- Journal of Chongqing Medical University Vol. 34, Issue 12, Pages: 1679-1682(2009)
- 作者机构：
  
  重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2009，
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[1]李智,戴立里.丹参素对肝星状细胞ERK1/2信号转导通路的影响[J].重庆医科大学学报,2009,34(12):1679-1682.

LI Zhi, et al. Effect of Danshengsu on ERK1/2 signal transduction pathway in hepatic stellate cell. [J]. Journal of Chongqing Medical University 34(12):1679-1682(2009)
[1]李智,戴立里.丹参素对肝星状细胞ERK1/2信号转导通路的影响[J].重庆医科大学学报,2009,34(12):1679-1682. DOI：

LI Zhi, et al. Effect of Danshengsu on ERK1/2 signal transduction pathway in hepatic stellate cell. [J]. Journal of Chongqing Medical University 34(12):1679-1682(2009) DOI：

摘要

目的:观察丹参素对血小板衍生生长因子-BB（Platelet derived growth factor-BB

PDGF-BB）刺激大鼠肝星状细胞（Hepatic stellate cell

HSC）增殖、Ⅰ型胶原合成、磷酸化细胞外信号调节激酶1/2（Phosphorylated extracellular signal-regulated kinase1/2

P-ERK1/2）表达的影响

探讨丹参素抗肝纤维化的分子机制。方法:四甲基偶氮唑盐法检测大鼠HSC增殖活性;免疫细胞化学染色法测定大鼠HSCⅠ型胶原合成的表达;Western blot测定大鼠肝星状细胞P-ERK1/2的表达。结果:丹参素有抑制PDGF-BB刺激大鼠HSC增殖

Ⅰ型胶原表达

P-ERK1/2表达的作用。结论:丹参素可抑制PDGF-BB刺激的大鼠HSC增殖

Ⅰ型胶原合成

其机制可能与抑制ERK1/2信号转导通路有关。

Abstract

Objective:To investigate the inhibitory effect of Danshengsu on proliferation

collagen typeⅠsynthesis and Phosphorylated extracellular signal-regulated kinase 1/2(P-ERK1/2)expression in rat hepatic stellate cell(HSC) stimulated by platelet derived growth factor-BB(PDGF-BB)

and to elucidate the molecular mechanism of Danshensu against hepatic fibrosis:Methods:Cell proliferation was evaluated by 3-(4

5-dimethylthiazol-2-y1)-2

5-Diphennyltetrazolium bromide(MTT)colorimetric assay.Collagen typeⅠsynthesis was detected by Immunocytochemical stain.Phosphorylated extracellular signal-regulated kinase 1/2(P-ERK1/2) expression was detected by Western blot.Results:Danshensu could inhibit proliferation

collagen typeⅠsynthesis and P-ERK1/2 expression of HSC stimulated by PDGF-BB.Conclusion:Danshensu can inhibit proliferation and Collagen typeⅠsynthesis of HSC stimulated by PDGF-BB

the mechanism of which may be Danshensu

s inhibitory effect on ERK1/2 signal transduction pathway.

关键词

丹参素肝星状细胞血小板衍生生长因子-BB细胞外信号转导激酶1/2

Keywords

DanshensuHepatic stellate cell(HSC)Pdatelet derivde gnowth factor-BB(PDGF-BB)Extracellular signal-regulated kinase 1/2(ERK1/2)

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