High frequency ultrasound in immunocompetent mouse model of xenograft neuroblastoma

LIU Qin1; WANG Qiao1; QUAN Xue-mo1; WANG Shan2; XIANG Juan2; BAI Jie2

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High frequency ultrasound in immunocompetent mouse model of xenograft neuroblastoma

更新时间：2024-01-17

- High frequency ultrasound in immunocompetent mouse model of xenograft neuroblastoma
- Journal of Chongqing Medical University Vol. 36, Issue 11, Pages: 1332-1335(2011)
- 作者机构：
  
  1. 重庆医科大学儿童医院超声科
  2. 重庆医科大学儿童医院肿瘤外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R445.1
- Published：2011，
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[1]刘沁,王荞,全学模,王珊,向娟,白洁.高频超声监测SD大鼠肾上腺区人神经母细胞瘤移植瘤模型[J].重庆医科大学学报,2011,36(11):1332-1335.

LIU Qin1, WANG Qiao1, QUAN Xue-mo1, et al. High frequency ultrasound in immunocompetent mouse model of xenograft neuroblastoma. [J]. Journal of Chongqing Medical University 36(11):1332-1335(2011)
[1]刘沁,王荞,全学模,王珊,向娟,白洁.高频超声监测SD大鼠肾上腺区人神经母细胞瘤移植瘤模型[J].重庆医科大学学报,2011,36(11):1332-1335. DOI：

LIU Qin1, WANG Qiao1, QUAN Xue-mo1, et al. High frequency ultrasound in immunocompetent mouse model of xenograft neuroblastoma. [J]. Journal of Chongqing Medical University 36(11):1332-1335(2011) DOI：

摘要

目的:探讨高频超声在SD大鼠肾上腺区人神经母细胞瘤（Neuroblastoma

NB）移植瘤模型中的应用价值

分析超声测量结果与肿瘤实际生长情况的相关性。方法:建立SD大鼠荷瘤模型53只。肿瘤接种后分别于5 d～6周

每4 d随机选取5只SD大鼠行高频超声检查

观察肿瘤的形态、回声和边界

测量肿瘤大小

彩色多普勒超声观察肿瘤内血流分布情况。每次超声检查后剥离肿瘤

测量肿瘤实际大小

并行病理活检

对比两者的相关性。结果:肿瘤成瘤率为100%;超声观察肾上腺区移植肿瘤呈类椭圆形中低回声团块

5～13 d肿瘤生长旺盛

其内血供丰富

>13～17 d生长速度减缓

>17 d肿瘤开始逐渐缩小

29 d后肿瘤内部出现液化坏死和钙化

血供减少;高频超声测量结果与肿瘤实际生长情况呈高度正相关（r=0.993

P<0.001）。结论:高频超声能较准确监测SD大鼠肾上腺区人NB移植瘤模型的建立及成瘤后肿瘤生长情况

具有较高的实用价值。

Abstract

Objective:To assess the value of high frequency ultrasound in monitoring the growth of xenograft neuroblastoma in immunocompetent sprague dawley(SD) mouse model.Methods:Fifty-three SD mice were implanted with human neuroblastoma(SK-N-SH) cell suspension.All of the mice were injected retroperitoneally in adrenal area.Five mice were chosen randomly and the size

internal echo and blood supply of tumor were observed by high frequency ultrasound at 5 d～6 weeks after the first injection.After the examination

the mice were dissected under narcotics and the tumors were dissociated immediately.The transversal and sagittal diameter was measured by high frequency ultrasound and straightedge.Tumors were fixed for HE staining.Results:The SD mouse model of xenograft neuroblastoma was made successfully

and the success rate was 100%.The volume of tumor measured by high frequency ultrasound and straightedge had strong correlation(r=0.993

P<0.001).High frequency ultrasound could also observe blood supply and necrosis of the tumors at real-time.The fast period of tumor growth with the richest blood supply appeared 5～13 days after injection.Conclusion:High frequency ultrasound provides an accurate

practical method to monitor the growth of xenograft neuroblastoma in SD mouse model.

关键词

彩色多普勒高频超声神经母细胞瘤大鼠

Keywords

ultrasonographycolor dopplerneuroblastomamouse

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Department of Surgical Oncology,Children’s Hospital of Chongqing Medical University,Key Laboratory of Pediatrics in Chongqing

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Department of VIP Clinic，the Children’s Hospital，Chongqing Medical University

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