Objective：to explore the way of Hp involvement in inflammation by analyzing the relationship between Hp infection and total white blood cell count and classification. Methods：Totally 7 665 patients were randomly selected from the health examination in 2016. All the subjects were tested by 13C urea expiratory test. According to the test results，the subjects were divided into 13C positive group（n=2 737） and 13C negative group（n=4 928），and the blood routine of all included subjects was also detected to explore the ap－plication of restricted cubic spline（RCS） in nonlinear regression. Restricted cubic spline was used to analyze the nonlinear relation－ship between age and 13C positive，chi-square test and analysis of variance were used to analyze differences of total white blood cell count and classification among groups. Results：Compared with those of subjects in the 13C negative group，the total white blood cells，the absolute value of neutrophils，the absolute value of lymphocytes and the absolute value of monocytes（P<0.05） were signifi－cantly higher in the 13C positive group. When delta over baseline（DOB） value was between 4 to 20，the age had significant effects on 13C positive（P=0.000），in which the women reached a peak at the age of 40 to 50，and men in their 60 years，but gender comparison between the two groups has no statistical significance（P>0.05）. Further analysis found that age was a risk factor for Hp infection（OR=1.012，95%CI=1.008 to 1.017）. Conclusion：Hp may participate in inflammatory response by activating neutrophils，lymphocytes and monocytes. Hp infection is not related to gender，and it has a parabolic relationship with age，and most importantly.