Objective：To investigate the association of rs2200733 and rs251253 polymorphisms with atrial fibrillation（AF）. Methods：We performed an association study between rs2200733 and rs251253 single nucleotide polymorphisms （SNPs） and atrial fibrillation. According to inclusion criteria，we enrolled 100 patients with AF，and the control group consisted of 107 patients without AF. Genomic DNA was extracted from blood samples of all patients using a TIANamp blood DNA kit according to the standard proto－col. The single nucleotide polymorphisms of rs2200733 and rs251253 were genotyped by polymerase chain reaction-restriction frag－ment length polymorphism（PCR-RFLP） analysis. Results：There was rs2200733 polymorphism in the case group.The frequencies of the three kinds of genotypes（TT，CT，CC） in AF group and control group were 21.0%，65.0%，14.0%；and 29.0%，46.7%，24.3%，re－spectively. The distribution of genotypes in AF group and control group had significant difference（P=0.027），with the frequency of CT genotype significantly higher in AF group than in control group（P=0.032）. No significant difference in the distributions of C and T al－lele frequencies was observed between two groups（P>0.05）. There was also rs251253 polymorphism in the case group. The frequen－cies of the three kinds of genotypes（GG，AG，AA） in AF group and control group were 70.0%，28.0%，2.0%；and 70.1%，25.2%，4.7%，respectively. There was no significant difference in distribution of genotypes and G and Aallele frequencies between two groups（P>0.05）. Conclusion：Polymorphism of rs2200733 may be associated with the risk of AF，and CT genotype may be a susceptible genet－ic factor for patients with atrial fibrillation.