Association of single nucleotide polymorphisms and combined genotypes of klotho G-395A，F352V and C370S with serum klotho protein levels in elderly people of Chinese Han population in Chongqing

ZHOU Ping; TIAN Xiaochun; MA Houxun; LIU Xiaolin; ZHAI Fuli; WU Ping; LI Faqi

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Association of single nucleotide polymorphisms and combined genotypes of klotho G-395A，F352V and C370S with serum klotho protein levels in elderly people of Chinese Han population in Chongqing

更新时间：2023-12-18

- Association of single nucleotide polymorphisms and combined genotypes of klotho G-395A，F352V and C370S with serum klotho protein levels in elderly people of Chinese Han population in Chongqing
- Journal of Chongqing Medical University Vol. 37, Issue 1, Pages: 21-24(2012)
- 作者机构：
  
  重庆医科大学附属第一医院老年病科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012，
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ZHOU Ping, TIAN Xiaochun, MA Houxun, et al. Association of single nucleotide polymorphisms and combined genotypes of klotho G-395A，F352V and C370S with serum klotho protein levels in elderly people of Chinese Han population in Chongqing. [J]. Journal of Chongqing Medical University 37(1):21-24(2012)
DOI：

ZHOU Ping, TIAN Xiaochun, MA Houxun, et al. Association of single nucleotide polymorphisms and combined genotypes of klotho G-395A，F352V and C370S with serum klotho protein levels in elderly people of Chinese Han population in Chongqing. [J]. Journal of Chongqing Medical University 37(1):21-24(2012) DOI：

摘要

目的：探讨重庆市汉族老年人群Klotho G-395A、F352V与C370S 3个位点的单核苷酸多态性（Single nucleotide poly－morphism，SNP）及其组合与血清klotho蛋白水平的相关性。方法：随机选择汉族老年人390例，采用等位基因特异性引物PCR技术检测Klotho G-395A、F352V与C370S SNP，酶联免疫吸附试验（Enzyme-linked immunosorbent assay，ELISA）检测其血清klotho蛋白水平。结果：Klotho G-395A、F352V与C370S SNP各等位基因型携带者其血清klotho蛋白水平均无显著性差异（P>0.05）。基因组合分析研究发现Klotho G-395A、F352V与C370S SNP各组合等位基因型携带者其血清klotho蛋白水平差异性较大，其中Klotho G-395G+F352F+C370S携带者较Klotho G-395G+F352F+C370C携带者其血清klotho蛋白水平明显增高（P=0.056）。结论：Klotho G-395A、F352V与C370S SNP各等位基因型携带者其血清klotho蛋白水平无显著性差异，而组合等位基因型研究发现Klotho G-395G+F352F+C370S携带者较Klotho G-395G+F352F+C370C组合等位基因型携带者血清klotho蛋白水平增高有显著性趋势。

Abstract

Objective：To investigate the association between serum klotho protein level and single nucleotide polymorphisms （SNPs） and combined genotypes of Klotho G-395A，F352V and C370S in elderly people of Han nationality in Chongqing. Methods：Unrelat－ed 390 elderly individuals from Chinese Han population （in Chongqing were selected.Single nucleotide polymorphisms of Klotho gene in the G-395A，F352V and C370S were tested by allele-specific primer（ASP） PCR，and serum klotho protein level was determined by enzyme-linked immunosorbent assay（ELISA）. Results：No significant difference was found in serum klotho protein level in the aged carrying allelic genotypes of Klotho G-395A，F352V and C370S SNP（P>0.05）. According to combined genotype analysis，the klotho protein level in the elderly individuals carrying Klotho G-395G+F352F +C370S was higher than that in the elderly individuals with Klotho G-395G+F352F+C370C（P=0.056）. Conclusion：No association of allelic genotypes of Klotho G-395A，F352V and C370S SNP with serum klotho protein levels is found.But a trend is found that serum klotho protein level in those with Klotho G-395G+F352F+C370S is higher than that in the elderly individuals with Klotho G-395G+F352F+C370C.

关键词

Klotho基因单核苷酸多态性组合基因型老年人

Keywords

klotho genesingle nucleotide polymorphismcombined genotypeelderly people

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