Objective：To observe changes in levels of serum hyaluronic acid（HA），type Ⅲ procollagen amino peptide（PⅢNP），laminin（LN） and collagen Ⅳ（CⅣ） in patients with liver cirrhosis and to explore the relationship between changes of above indexes and de－grees of liver fibrosis. Methods：Blood samples of 204 patients with liver disease were collected in our center from January 2011 to September 2012. The 204 patients（experimental group） included 124 males and 80 females，aged from 26 to 67，48 in average；44 patients with mild chronic hepatitis，50 with moderate chronic hepatitis，31 with severe chronic hepatitis and 79 with posthepatitic cirrhosis. Forty-six health controls from staffs and physical examination persons in our center were enrolled as normal control group，including 25 males and 21 females，aged from 22 to 52，38 in average. Levels of HA，PⅢNP，LN and CⅣ were detected by γ-radia－tion immunity counter. Differences in levels of the indexes between each experimental group and normal control group were analyzed. Results：Levels of HA，PⅢNP，LN and CⅣ were elevated in each experimental group than in normal control group，with statistically significant differences（P<0.01）. Moreover，these indexes were increased gradually followed by the development of disease and the in－crease of HA levels was the most obvious. Conclusions：Serum levels of HA，PⅢNP，LN and CⅣ in patients could be used to indicate the occurrence of liver fibrosis and the degree of fibrosis and could also be used as one of the prognostic indicators after treatment.