Objective：To discuss the clinical significance of amniotic membrane transplantation combined with mitomycin C in the treatment of symblepharon after severe eye burns. Methods：Twenty-seven patients（27 eyes） with severe symblepharon were treated with symblepharon resection then were transplanted with preserved human amniotic membrane combined with mitomycin C for bulbar conjunctiva and corneal surface reconstruction. Results：Symblepharon of 27 eyes was improved after operation，21 eyes（77.8%） ob－tained fine results；6 eyes（22.2%） had slight symblepharon recurrence，but were improved obviously compared with that of pre-opera－tion，no invalid case was found. Conclusion：Amniotic membrance transplantation combined with mitocmycin C is an ideal method in the treatment of symblepharon after severe eye burns and it is worthy to be popularized.