Expression of PRDX2 in colorectal neoplasms and its mechanism

Wu Xingye; Liu Maoxi; Du Kunli; Fu Zhongxue

您当前的位置：

首页 >

文章列表页 >

Expression of PRDX2 in colorectal neoplasms and its mechanism

更新时间：2023-12-18

- Expression of PRDX2 in colorectal neoplasms and its mechanism
- Journal of Chongqing Medical University Vol. 38, Issue 12, Pages: 1732-1735(2014)
- 作者机构：
  
  重庆医科大学附属第一医院胃肠外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2014，
扫描看全文
Wu Xingye, Liu Maoxi, Du Kunli, et al. Expression of PRDX2 in colorectal neoplasms and its mechanism. [J]. Journal of Chongqing Medical University 38(12):1732-1735(2014)
DOI：

Wu Xingye, Liu Maoxi, Du Kunli, et al. Expression of PRDX2 in colorectal neoplasms and its mechanism. [J]. Journal of Chongqing Medical University 38(12):1732-1735(2014) DOI：

摘要

目的：探讨过氧化氧化还原蛋白2 （peroxiredoxins 2，PRDX2）在结直肠癌及正常结直肠组织中的表达情况及其可能的作用机制。方法：收集32例组织标本，采用免疫组织化学及Western blot的方法对PRDX2在结直肠癌组织及正常结直肠组织中的表达情况进行检测；免疫共沉淀及HPLC-CHIP-MS/MS方法检测结肠癌细胞SW480中与PRDX2相互作用的蛋白质。结果：免疫组织化学显示：PRDX2蛋白主要表达于细胞浆，在结直肠正常组织（21.88%，7/32）与结直肠癌组织中（71.88%，23/32）PRDX2蛋白的表达差异有统计学意义（P=0.001）。随机抽取8例标本进行Western blot的检测，PRDX2蛋白在7例癌组织中表达高于正常组织，差异有统计学意义（P<0.01）。在结肠癌细胞SW480中鉴定出17种与PRDX2相互作用的蛋白质，其中有9种蛋白受c-Myc基因的调控。结论：PRDX2在结直肠癌的发展、转移中发挥了一定的作用；PRDX2可能与c-Myc基因调控通路间存在密切的联系。

Abstract

Objective：To investigate the expression of PRDX2 in histological samples of colorectal neoplasm and the normal tissues and to analyze its mechanism. Methods：The expression of PRDX2 was detected by immunohistochemistry and Western blot. In colon cancer cell SW480，interacting proteins with PRDX2 was detected by immunoprecipitation and HPLC-CHIP-MS/MS. Results：Im－munohistochemistry indicated that PRDX2 protein was expressed mainly in the cytoplasm. The expression of PRDX2 was weaker in normal colorectal tissues（21.88%，7/32） than in carcinomas tissue（71.88%，23/32）（P=0.001）. Western blot analysis revealed that the expression of PRDX2 protein was significantly higher in carcinomas than in control colorectal tissues（P<0.01）. In SW480 cells，17 proteins were obtained from immunoprecipitation using specific antibodies against PRDX2，9 of them were regulated by c-Myc gene. Conclusion：PRDX2 play roles in the development and metastasis of colorectal cancer. PRDX2 might be associated with c-Myc gene regulatory pathway.

关键词

结直肠肿瘤过氧化氧化还原蛋白2c-Myc

Keywords

colorectal neoplasmsperoxiredoxins 2c-Myc

references

Views

114

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of beta-catenin,protein APC and c-myc,cyclin D₁ in Ovarian epithelial tumor and its significance

The study on platelet-derived growth factor and c-myc together inhibiting the stenosis of transplanted vascular

Related Author

LIN Xiao

et al

张雄

XIANG Zhi

et al

吴忠均

Related Institution

Department of Pathology,College of Basic Medicine,Chongqing Medical University

重庆医科大学基础医学院病理教研室

Departmen of General Surgery,the First Affiliated Hospital,Chongqing Medical University

⁰