Objectives：To establish the reference intervals of commonly used liver and renal function test items among healthy preschool and school-aged children in Chongqing and to provide better references for clinical diagnosis and treatment. Methods：The stratified randomized cluster sampling method was used to select 545 healthy preschool（1-6 years old） and school-aged（7-12 years old）children（male：320，female：225）. Thirteen commonly used liver and renal function test items in venous blood were detected by the HITACHI 7600；data were analyzed by statistical method. Normal distribution data were used to calculate the reference intervals ac－cording to x±1.96 s and skewness distribution data were used to calculate the reference intervals according to P2.5-P97.5. Results：（1）The population means of liver and renal function test items between healthy preschool and school-aged children in Chongqing were significantly different except the blood urea nitrogen（BUN） item. （2）The population means of total bilirubin（TB），direct bilirubin（DB），total protein（TP），albumin（ALB），alanine aminotransferase（ALT），aspertate aminotransferase（AST），alkaline phosphatase（ALP），gamma-glutamyl transpeptidase（GGT），lactate dehy－drogenase（LDH），BUN，creatinine（CREA） and uric acid（UA） showed no significant difference except blood glucose（GLU） between dif－ferent genders among preschool children. The 95% reference intervals of serum TB，DB，TP，ALB，ALT，AST，ALP，GGT，LDH，BUN，CREA and UA were 5.4-18.9 μmol/L，0－6.7 μmol/L，58-71 g/L，42-50 g/L，8.7-26 U/L，21-41 U/L，112-312 U/L，7.1-15.8 U/L，190-312 U/L，2.3-6.4 μmol/L，14-42 μmol/L，156-366 μmol/L respectively. The 95% reference intervals of serum GLU based on gender were 3.7-6.0 mmol/L for male and 3.4~5.8 mmol/L for female. （3）The population means of Glu，TB，DB，TP，ALB，AST，ALP，LDH，CREA and UA showed no significant difference between different genders among school-aged children except ALT，GGT and BUN. The 95% reference intervals of GLU，TB，DB，TP，ALB，AST，ALP，LDH，CREA and UA were 4.2-6.0 mmol/L，6.6－23.6 μmol/L，0-6.8 μmol/L，60-76 g/L，43-52 g/L，18-36 U/L，116~366 U/L，162-293 U/L，22-51 μmol/L，174-401 μmol/L respectively. The 95% reference intervals based on gender were established as ALT：8.9-28 U/L for male，7.2-29.5 U/L for female；GGT：8.8-21 U/L for male，6.1-22.5 U/L for female；BUN：2.8-7.0 μmol/L for male，2.2-6.2 μmol/L for female. Conclusion：The reference intervals of thirteen commonly used liver and renal function test items are established by Hitachi 7600 system among healthy preschool and school-aged children in Chongqing；it can provide accurate experimental basis for clinical diagnosis and treatment in children.