Objective To explore the variation of ryanodine receptor subtype 2 (ryr2) opening and its mechanism in detrusor overactivity(DO). Methods An animal model of overactive detrusor muscle of the bladder was constructed. The spontaneous calcium activity in the normal control group and DO group was detected by laser scanning confocal microscope

and the expression of phosphorylated RyR2(p-RyR2) and FKBP12.6 in the two groups were detected by Western blot. Results Compared with the normal control group

the DO group showed significantly decreased spontaneous calcium activity

increased expression of FKBP12.6 protein

and decreased expression of p-RyR2. Conclusion In the DO group

the expression of FKBP12.6 protein was significantly increased

and the expression of pRyR2 was significantly decreased

which may be the key reason for the reduction of the RyR2 opening in the detrusor overactivity.