Study on the mechanism of hypoxia-induced IL-11 secretion in osteosarcoma cell line U2OS

YANG Tengjiao; LI Hujin; WANG Pan; FENGYong

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Study on the mechanism of hypoxia-induced IL-11 secretion in osteosarcoma cell line U2OS

更新时间：2023-05-22

- Study on the mechanism of hypoxia-induced IL-11 secretion in osteosarcoma cell line U2OS
- null Issue 9, Pages: 691(2019)
- 作者机构：
  
  1. 重庆大学附属肿瘤医院，肿瘤转移与个体化诊治转化研究重庆市重点实验室,重庆,400030
  2. 重庆市急救医疗中心骨科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2019，
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YANG Tengjiao, LI Hujin, WANG Pan, et al. Study on the mechanism of hypoxia-induced IL-11 secretion in osteosarcoma cell line U2OS. [J]. null (9):691(2019)
DOI：

YANG Tengjiao, LI Hujin, WANG Pan, et al. Study on the mechanism of hypoxia-induced IL-11 secretion in osteosarcoma cell line U2OS. [J]. null (9):691(2019) DOI：

摘要

目的探讨缺氧诱导骨肉瘤U2OS细胞分泌IL-11的分子机制。方法体外缺氧（1%O2）环境下培养人骨肉瘤U2OS细胞系，同时加入HIF-1α抑制剂孵育，或预先加入不同信号通路抑制剂处理，Western blot检测HIF-1α的蛋白水平，定量PCR检测IL-11的mRNA水平，ELISA检测IL-11的蛋白水平。结果与正常氧环境培养的U2OS细胞相比，缺氧显著诱导U2OS细胞上调HIF-1α的表达，同时促进IL-11的mRNA水平和蛋白水平增加，并具有时间依赖性；加入HIF-1α抑制剂后可显著抑制缺氧诱导的HIF1α表达以及IL11的mRNA和蛋白水平；加入JNK信号抑制剂亦显著抑制缺氧诱导的IL-11表达和分泌，上述差异均具有统计学意义(P＜0.05)。但HIF-1α的表达无显著变化，差异无统计学意义（P＞0.05）。结论缺氧可通过HIF-1α-JNK信号诱导骨肉瘤U2OS细胞分泌IL-11，从而促进骨肉瘤的发生发展。

Abstract

Objective To study the mechanism of hypoxia-induced IL-11 secretion in osteosarcoma cell line U2OS.Methods U2OS cells were cultured （1%O2） in vitro under hypoxia condition with the hypoxia-inducible factor-1α （HIF-1α） inhibitor or different signal pathway inhibitors preincubated in advance.The protein level of HIF-1α was detected by Western blot

and the mRNA level of IL-11 was detected by RT-PCR

and the protein level of IL-11 was detected by enzymelinked immunosorbent assay (ELISA). Results Under hypoxia condition

the expression of HIF-1α was significantly increased

and the mRNA and protein levels of IL11 were also significantly increased (P<0.05)

which is in a time dependent manner.In addition

the hypoxiainduced HIF-1α expression and the mRNA and protein levels of IL-11 were significantly inhibited when cells were incubated with the HIF-1α inhibitor (P＜0.05).When cells were pretreated with JNK signal inhibitor

the expression and secretion of IL-11 were significantly inhibited (P＜0.05) while the expression of HIF-1α was not altered (P＞0.05). Conclusion Hypoxia could induce the secretion of IL-11 by U2OS cells via HIF-1α-JNK signal pathway

which may be involved in the development of osteosarcoma.

关键词

缺氧IL-11HIF-1αJNK骨肉瘤

Keywords

hypoxiaIL-11HIF-1αJNKosteosarcoma

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