Inhibitory effect of Morinda officinalis water extract on testicular cell apoptosis induced by varicocele in SD rats

LIN Wendong; DING Xiaoming; LI Deshui

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Inhibitory effect of Morinda officinalis water extract on testicular cell apoptosis induced by varicocele in SD rats

更新时间：2023-05-22

- Inhibitory effect of Morinda officinalis water extract on testicular cell apoptosis induced by varicocele in SD rats
- null Issue 9, Pages: 687(2019)
- 作者机构：
  
  1. 泉州医学高等专科学校动物实验室,福建,泉州,362011
  2. 泉州医学高等专科学校基础医学部解剖教研室,福建,泉州,362011
- 作者简介：
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  CLC：
- Published：2019，
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LIN Wendong, DING Xiaoming, LI Deshui. Inhibitory effect of Morinda officinalis water extract on testicular cell apoptosis induced by varicocele in SD rats. [J]. null (9):687(2019)
DOI：

LIN Wendong, DING Xiaoming, LI Deshui. Inhibitory effect of Morinda officinalis water extract on testicular cell apoptosis induced by varicocele in SD rats. [J]. null (9):687(2019) DOI：

摘要

目的分析巴戟天水提取物对精索静脉曲张（VC）大鼠睾丸组织Livin和Smac的表达及意义。方法选取30只雄性SD大鼠分为假手术组、模型组和观察组

每组10只。模型组及观察组构建精索静脉曲张（VC）模型，假手术组不做结扎；假手术组采用生理盐水干预，模型组造模后生理盐水灌胃，观察组造模后巴戟天水提取物灌胃；术前及术后称重，术后检测大鼠睾丸及附睾指数；取出左侧睾丸检测切片中Livin、Smac表达情况及细胞凋亡情况。结果造模前及造模后3组大鼠体质量检测结果差异无统计学意义（P>0.05）；大鼠睾丸指数及附睾指数，观察组均显著高于模型组

差异有统计学意义（P<0.05），但观察组与假手术组差异无统计学意义（P>0.05）；大鼠睾丸组织凋亡指数，观察组显著低于模型组，且模型组显著高于假手术组，差异有统计学意义（P<0.05）；大鼠睾丸组织Livin阳性细胞率及平均光密度，观察组均显著高于模型组，且显著低于假手术组，差异有统计学意义（P<0.05）；大鼠睾丸组织Smac阳性细胞率及平均光密度，观察组均显著低于模型组，且显著低于假手术组，差异有统计学意义（P<0.05）。结论采用巴戟天水提取物对VC SD大鼠干预后，可有效改善大鼠睾丸组织Livin和Smac表达。

Abstract

Objective To analyze the expression and significance of Morinda officinalis water extract on Livin and Smac in testicular tissue of varicocele (VC) rats.Methods Selected 30 male SD rats and divided them into the sham operation group

the model group

and the observation group

with 10 rats in each group. VC model was constructed in the model group and the observation group

and no ligation was performed in the sham operation group. Normal saline was used in the sham operation group

normal saline was given to the model group after modeling

and the observation group was given the Morinda officinalis water extract after modeling. The indexes of testicles and epididymis were measured after operation. Extracted the left testicle to detect the expression of Livin and Smac

and to detect the cell apoptosis.Results There was no significant difference in body weigh among the three groups before and after modeling (P>0.05). The testicular index and epididymal index in the observation group were significantly higher than those in the model group

and the difference was statistically significant (P<0.05); but there was no statistically significant difference between the observation group and the sham operation group (P>0.05). The apoptosis index of testicular tissue in the observation group was significantly lower than that of the model group

and the difference was statistically significant (P<0.05); the model group was significantly higher than that of the sham operation group

and the difference was statistically significant (P<0.05). The positive cells rate and average optical density of Livin in testicular tissue in the observation group were significantly higher than those in the model group

and they were significantly lower than those in the sham operation group

with statistically significant differences (P<0.05).The rate of positive cells and average optical density of Smac in testicular tissue in the observation group were significantly lower than those in the model group

and they were significantly lower than those in the sham operation group

with statistically significant differences (P<0.05). Conclusion The Livin and Smac expressions in testicular tissue of varicocele SD rats could be effectively improved after the intervention of the Morinda officinalis water extract.

关键词

巴戟天精索静脉曲张睾丸组织LivinSmac细胞凋亡

Keywords

Morinda officinalisvaricoceletesticular tissueLivinSmacapoptosis

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