Traceability analysis and drug sensitivity testing of a food poisoning caused by Salmonella
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Traceability analysis and drug sensitivity testing of a food poisoning caused by Salmonella
Health Medicine Research and PracticeVol. 19, Issue 1, Pages: 42-45(2022)
作者机构:
济南市疾病预防控制中心细菌性疾病检验所
作者简介:
基金信息:
DOI:
CLC:R155.3
Published:2022,
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Li Na, Jiang Huiyu, Shi Yuwen, et al. Traceability analysis and drug sensitivity testing of a food poisoning caused by Salmonella. [J]. Health Medicine Research and Practice 19(1):42-45(2022)
DOI:
Li Na, Jiang Huiyu, Shi Yuwen, et al. Traceability analysis and drug sensitivity testing of a food poisoning caused by Salmonella. [J]. Health Medicine Research and Practice 19(1):42-45(2022)DOI:
Traceability analysis and drug sensitivity testing of a food poisoning caused by Salmonella
Objective To perform pulsed field gel electrophoresis(PFGE) molecular typing tracing and drug resistance analysis on a food poisoning caused by Salmonella contaminated chicken claw
in order to provide relevant theoretical basis for identifying the source of this food poisoning and clinical treatment of patients.Methods Epidemiological investigation was conducted on the six patients
and specimens of suspected food and stool were collected and laboratory tests were performed according to national standards.After bacterial enrichment
the suspicious colonies were picked for biochemical identification
and the seven strains of Salmonella detected underwent serum agglutination with standard diagnostic sera
and the DNA of the strains were cut by restriction endonuclease Xba Ⅰ and then subjected to PFGE molecular typing.The results were clustered by Bio Numerics software
and finally the seven strains of Salmonella were tested for drug resistance using the micro broth dilution method.Results All 6 patients had different degrees of abdominal pain
diarrhea and fever;1 strain of Salmonella was detected from chicken claw and 6 strains of Salmonella were detected in the patient’s stool specimen.The biochemical identification and serum agglutination test showed that the serotypes of the seven Salmonella strains were all the Fallsbüttel serotype
PFGE showed that the homology of the seven Salmonella strains was 100.0%
and the drug sensitivity results showed that all seven strains were resistant to tetracyclines(tetracycline
minocycline) and macrolides(azithromycin).Conclusion The incident of mass diarrhea
fever and abdominal pain was a case of food poisoning caused by Salmonella.The relevant departments should increase the publicity and popularization of food safety knowledge to raise public awareness;the public should try to heat and process the purchased cooked food before consumption
which is an important measure to prevent Salmonella food poisoning.At the same time
it is recommended that the regulatory authorities should strengthen the supervision of small food workshops
restaurants and other production and selling places to prevent similar incidents.