Objective To explore the diagnostic value of serum sex hormones in female reproductive infertility. Methods Sixty patients with infertility treated in a hospital in Henan Province from January 2018 to August 2020 were selected as the study group

and 60 healthy women of childbearing age who were in hospital for physical examination at the same time were selected as the control group. The 3 mL elbow venous blood samples in the morning from 2 groups were collected for electrochemiluminescence immunoassay（ECLIA）. The detection indexes included follicle stimulating hormone（FSH）

estradiol（E

2

）

progesterone（P）

prolactin（PRL）

luteinizing hormone（LH） and testosterone（T）. Results The levels of FSH

LH

PRL and T in the study group were higher than those in the control group

while the levels of P and E

2

in the study group were lower than those in the control group. The differences were statistically significant（P

<

0.05）. Conclusion The change of sex hormone level can be clinically used as the diagnostic basis of female infertility.