Effects of fasudil on proliferation,migration and extracellular matrix synthesis of urethral fibroblasts induced by TGF-β

FENG Huan; HUANG Xiaobing; FU Weihua; ZHU Jingzhen; LI Jia; YANG Fengxia

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Effects of fasudil on proliferation,migration and extracellular matrix synthesis of urethral fibroblasts induced by TGF-β

更新时间：2022-06-24

- Effects of fasudil on proliferation,migration and extracellular matrix synthesis of urethral fibroblasts induced by TGF-β
- null Issue 6, Pages: 463(2021)
- 作者机构：
  
  陆军军医大学第二附属医院泌尿外科中心,重庆,400037
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FENG Huan, HUANG Xiaobing, FU Weihua, et al. Effects of fasudil on proliferation,migration and extracellular matrix synthesis of urethral fibroblasts induced by TGF-β. [J]. null (6):463(2021)
DOI：

FENG Huan, HUANG Xiaobing, FU Weihua, et al. Effects of fasudil on proliferation,migration and extracellular matrix synthesis of urethral fibroblasts induced by TGF-β. [J]. null (6):463(2021) DOI：

摘要

目的探究法舒地尔对TGFβ诱导尿道成纤维细胞增殖、迁移以及胞外基质合成的影响。方法无菌条件下获得家兔尿道瘢痕组织，采用酶解法提取原代尿道成纤维细胞，用10 ng/mL TGF-β或者联合不同浓度（0，25，50 μM）法舒地尔处理后，通过Western blot检测α-SMA、胶原蛋白Ⅰ、胶原蛋白Ⅲ和自噬以及迁移相关蛋白的表达。采用Transwell实验和MTT实验分别检测成纤维细胞迁移以及增殖能力。结果 Western blot结果显示

TGF-β明显上调了α-SMA蛋白、胶原蛋白Ⅰ、胶原蛋白Ⅲ，法舒地尔下调了胞外基质蛋白表达（P＜0.05），并且呈浓度依赖性。Transwell实验和MTT实验显示，TGFβ增加了细胞迁移数量和细胞活性（P＜0.05），而法舒地尔抑制了细胞迁移数量（P＜0.05），下调了细胞活性（P＜0.05）。Western blot结果还显示，TGF-β显著下调了pAKT、pmTOR和p62等蛋白表达（P＜0.05），上调了ATG7、ATG5、LC3等自噬相关蛋白表达（P＜0.05）；而法舒地尔上调了pAKT、pmTOR和p62等蛋白表达（P＜0.05），抑制了ATG7、ATG5、LC3等自噬相关蛋白表达（P＜0.05）。结论法舒地尔可以通过激活细胞自噬从而抑制TGFβ诱导的成纤维细胞增殖、迁移以及胞外基质合成，其作用与促进AKT/mTOR信号通路相关。

Abstract

关键词

法舒地尔尿道成纤维细胞TGF-β自噬尿道狭窄

Keywords

fasudilurethral fibroblastsTGF-βautophagyurethrostenosis

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