Mechanism of transforming growth factor-β1 induced the secretion of vascular endothelial growth factor C in gastric cancer cell line AGS

TIAN Yin; HU Wenjing; SHANG Yangyang; WU Yun; LIU Yun

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Mechanism of transforming growth factor-β1 induced the secretion of vascular endothelial growth factor C in gastric cancer cell line AGS

更新时间：2022-06-24

- Mechanism of transforming growth factor-β1 induced the secretion of vascular endothelial growth factor C in gastric cancer cell line AGS
- null Issue 3, Pages: 180(2020)
- 作者机构：
  
  陆军军医大学第一附属医院消化内科,重庆,400038
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TIAN Yin, HU Wenjing, SHANG Yangyang, et al. Mechanism of transforming growth factor-β1 induced the secretion of vascular endothelial growth factor C in gastric cancer cell line AGS. [J]. null (3):180(2020)
DOI：

TIAN Yin, HU Wenjing, SHANG Yangyang, et al. Mechanism of transforming growth factor-β1 induced the secretion of vascular endothelial growth factor C in gastric cancer cell line AGS. [J]. null (3):180(2020) DOI：

摘要

目的探讨转化生长因子β1(TGF-β1)诱导胃癌细胞AGS分泌血管内皮生长因子C(VEGF-C)的分子机制。方法体外培养胃癌细胞系AGS细胞，将其分为未刺激组、TGF-β1刺激组、TGF-βR抑制剂组、DMSO组、Smad3抑制剂组、NF-κB抑制剂组、MEK1/2抑制剂组、p38/MAPK抑制剂组以及JNK抑制剂组。除未刺激组外，其余各组均用TGF-β1刺激细胞，并用酶联免疫吸附试验（ELISA）检测细胞培养上清液中VEGF-C的水平。结果 TGF-β1刺激组的VEGF-C分泌水平显著高于未刺激组，差异有统计学意义(P<0.05)，TGF-β1能够诱导AGS细胞分泌VEGF-C，并具有剂量和时间依赖性。在TGF-β1刺激的条件下，TGF-βR抑制剂组及Smad3抑制剂组AGS细胞分泌的VEGF-C水平均显著低于DMSO组，差异有统计学意义(P<0.01)；而NF-κB、MEK1/2、p38/MAPK和JNK抑制剂组中VEGF-C水平与DMSO组比较差异则无统计学意义（P＞0.05）。结论 TGF-β1可通过其受体及下游的Smad3信号通路诱导AGS细胞分泌VEGF-C，从而有助于胃癌的淋巴转移。

Abstract

关键词

转化生长因子β1血管内皮生长因子CSmad3胃癌AGS分子机制

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