Dioscin inhibits IL-1β-induced catabolism and apoptosis by regulating the TLR4/NF-κB signaling pathway in human nucleus pulposus cells

GAO Weisong; WANG Longhui; GU Yuntao; ZHAO Hai; QI Hao; WU ChangXin

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Dioscin inhibits IL-1β-induced catabolism and apoptosis by regulating the TLR4/NF-κB signaling pathway in human nucleus pulposus cells

更新时间：2022-06-24

- Dioscin inhibits IL-1β-induced catabolism and apoptosis by regulating the TLR4/NF-κB signaling pathway in human nucleus pulposus cells
- null Issue 10, Pages: 857(2021)
- 作者机构：
  
  海南医学院第二附属医院骨科,海南,海口,570102
- 作者简介：
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GAO Weisong, WANG Longhui, GU Yuntao, et al. Dioscin inhibits IL-1β-induced catabolism and apoptosis by regulating the TLR4/NF-κB signaling pathway in human nucleus pulposus cells. [J]. null (10):857(2021)
DOI：

GAO Weisong, WANG Longhui, GU Yuntao, et al. Dioscin inhibits IL-1β-induced catabolism and apoptosis by regulating the TLR4/NF-κB signaling pathway in human nucleus pulposus cells. [J]. null (10):857(2021) DOI：

摘要

目的探讨薯蓣皂苷(Dio)对白细胞介素-1β（IL-1β）诱导的人髓核（NP）细胞炎症反应的作用及机制。方法将人NP细胞分为control组、IL-1β组、IL-1β+Dio 100 ng?mL-1组和IL-1β+Dio 500 ng?mL-1组，用IL-1β和不同浓度的Dio分别进行体外培养。采用CCK-8法、Western blot、免疫荧光染色、Griess法、ELISA和RT-PCR检测细胞活力、细胞外基质代谢情况、炎症因子及相关信号传导途径。结果 Dio能够抑制IL-1β刺激下人NP细胞的PGE2、NO、iNOS和COX-2表达(P＜0.05），抑制人NP细胞IL-1β激活的分解代谢活性(P＜0.05），抑制IL-1β刺激的人NP细胞TLR4/NF-κB信号通路(P＜0.05），降低炎症介质（IL-6、TNF-α）水平(P＜0.05）。结论 Dio通过抑制TLR4/NF-κB信号通路以及相关的炎症反应，抑制IL-1β诱导的人NP细胞炎症和分解代谢活性，为临床治疗椎间盘退变（IDD）提供了新的证据。

Abstract

关键词

细胞凋亡分解代谢薯蓣皂苷炎症椎间盘退变TLR4/NF-κB

Keywords

apoptosiscatabolismdioscininflammationintervertebral disc degenerationTLR4/NF-κB

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