WANG Yuanyuan, ZHAO Wenmei, LI Ke, et al. Establishment and application of an analytical method for the main active ingredients in longan. [J]. Journal of Southwest Medical University 44(2):114-119(2021)
WANG Yuanyuan, ZHAO Wenmei, LI Ke, et al. Establishment and application of an analytical method for the main active ingredients in longan. [J]. Journal of Southwest Medical University 44(2):114-119(2021)DOI:
Establishment and application of an analytical method for the main active ingredients in longan
Objective: To establish a simple and accurate method for the analysis of main active components in longan. Methods: An analytical method based on ultraviolet spectrophotometry was established to measure the content of total sugar
total flavonoids
and total polyphenols in Luzhou longan wine. Results: An analytical method for the main active ingredients in longan was successfully established based on ultraviolet spectrophotometer. The regression equations of major components had R
2
values greater than 0.999
indicating that the linear fitting of each component was reliable. The precision values expressed as relative standard deviation were less than 2%
and the recoveries were 80%~120%
which met the requirements of analysis. The content of main ingredients in longan wine was successfully determined by this method. The total sugar content of Y-3
Y-4
Y-5
and Y-6 was 20.94
49.01
39.70
and 47.61 mg/mL
respectively. The total polyphenol content of Y-3
Y-4
Y-5
and Y-6 was 0.597
0.291
0.473
and 0.555 mg/mL
respectively. The total flavonoid content of Y-3
Y-4
Y-5
and Y-6 was 69.58
50.41
292.92
and 387.45 μg/mL
respectively. Conclusion: This method can be used for determination of the main components in longan.