Effect of HtrA on the fructosyltransferase expression in cariogenic Streptococcus mutans

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Effect of HtrA on the fructosyltransferase expression in cariogenic Streptococcus mutans

更新时间：2023-04-14

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- Effect of HtrA on the fructosyltransferase expression in cariogenic Streptococcus mutans
- Journal of Southwest Medical University Vol. 43, Issue 5, Pages: 446-449(2020)
- 作者机构：
  
  1. 西南医科大学口颌面修复重建与再生实验室
  2. 西南医科大学附属口腔医院儿童口腔科
  3. 四川绵阳四0四医院口腔科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R781.1
- Published：2020，
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[1]毛玲,杨维虹,徐仕珍,王玲,韩林秀,刘兴容.高温需要蛋白A对乳牙变异链球菌果糖基转移酶表达的影响[J].西南医科大学学报,2020,43(05):446-449.

MAO Ling, YANG Weihong, XU Shizhen, et al. Effect of HtrA on the fructosyltransferase expression in cariogenic Streptococcus mutans. [J]. Journal of Southwest Medical University 43(5):446-449(2020)
[1]毛玲,杨维虹,徐仕珍,王玲,韩林秀,刘兴容.高温需要蛋白A对乳牙变异链球菌果糖基转移酶表达的影响[J].西南医科大学学报,2020,43(05):446-449. DOI：

MAO Ling, YANG Weihong, XU Shizhen, et al. Effect of HtrA on the fructosyltransferase expression in cariogenic Streptococcus mutans. [J]. Journal of Southwest Medical University 43(5):446-449(2020) DOI：

摘要

目的:研究乳牙高致龋性变异链球菌（streptococcus mutans

S.mutans）高温需要蛋白A（high temperature requirement serine proteinase A

HtrA）基因缺陷株和HtrA高毒力株在体外非应激环境中的果糖基转移酶（fructosyltransferase

FTF）表达能力的差异。方法:选用课题前期已获得的HtrA高毒力株及以此构建的HtrA基因缺陷株、S.mutans国际标准株UA159在脑心浸出液肉汤（brain heart infusion broth

BHI）培养基培养16 h后

用实时荧光定量核酸扩增检测系统（real-time quantitative PCR detecting system

RT-qPCR）

检测三菌株FTF基因的扩增情况。用相对定量分析-2-△△Ct法计算并比较HtrA基因缺陷株与HtrA高毒力株和UA159标准株FTF基因的相对表达能力的差异。结果:HtrA高毒力株的FTF基因的表达量约是HtrA基因缺陷株23.588倍;UA159标准株的FTF基因的表达量约是HtrA基因缺陷株10.556倍。结论:乳牙变异链球菌的FTF的表达受到HtrA的调控。

Abstract

Objective: To investigate the difference in the expression ability of fructosyltransferase（FTF） in vitro in a non-stressful environment between a high temperature requirement serine proteinase A（HtrA） gene-deficient Streptococcus mutans strain and a HtrA high-virulent S. mutans strain with high cariogenicity. Methods: A high-virulent S. mutans strain obtained in our previous study

an HtrA gene-deficient strain constructed based on it

and an international standard strain

S. mutans UA159

were selected and cultured in brain-heart infusion（BHI）broth for 16 h. The real-time quantitative PCR detection system was used to determine the amplification of FTF gene in the three strains. The relative quantitative analysis—2-△△Ctmethod was used to calculate and compare the relative expression of FTF gene in the three strains. Results: The FTF gene expression in the HtrA high-virulent strain was23.588 times that in the HtrA gene-deficient strain

while that in the UA159 standard strain was 10.556 times that in the HtrA gene-deficient strain. Conclusion: The expression of FTF in cariogenic S. mutans is regulated by HtrA.

关键词

乳牙龋病变异链球菌高温需要蛋白A果糖基转移酶

Keywords

Deciduous teethDental cariesStreptococcus mutansHigh temperature requirement serine proteinase A（HtrA）Fructosyltransferase（FTF）

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