Objective: To establish a method based on multichannel high-performance liquid chromatography（HPLC） for simultaneous determination of the content of gentiopicroside

geniposide

baicalin

and ammonium glycyrrhizinate in Longdanxiegan capsules. Methods: HPLC was performed on a Kromasil 100-5 C18 column（125 mm ×4.0 mm

5 μm） at 30 ℃ using the mobile phase of acetonitrile（A）-0.1% phosphoric acid（B） for gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was 238 nm for geniposide

270 nm for gentiopicroside

280 nm for baicalein

and 250 nm for ammonium glycyrrhizinate. The injection volume was 10 μL. Results: There was a good linear relationship between concentration and peak area for geniposide within 8.526 ～ 170.52 μg/mL

for gentiopicroside within 3.774 ～ 75.48 μg/mL

for baicalin within 6.867～137.34 μg/mL

and for ammonium glycyrrhizinate within 1.66 ～ 33.16 μg/mL（r >0.999）. The average recoveries were 101.08%（relative standard deviation [RSD] =1.73%）

101.10%（RSD = 1.81%）

98.48%（RSD = 1.21%）

and 101.47%（RSD = 1.34%）

respectively. There was good precision

stability

and repeatability

with RSD less than 2%. Conclusion: The established method is simple

rapid

and accurate

and can be used for the quality control of Longdanxiegan capsules and subsequent improvement of quality standards.