Objective: To simultaneously determine the content of eugenol

linalool

and eucalyptol in laurel oil by high-performance liquid chromatography（HPLC）. Methods: HPLC was performed on a Dikma Diamonsil C18 column（250 mm × 4.6 mm

5 μm） with a mobile phase of acetonitrile-methanol-water（35∶29∶36） at a detection wavelength of 200 nm

a column temperature of 30 °C

and a flow rate of 1.0 mL/min. Results: The concentrations of eugenol

linalool

and eucalyptol in laurel oil showed good linear relationships with the corresponding peak areas in the ranges of 0.021 ～ 0.208 mg/mL（r = 0.9999）

0.048 ～ 0.481 mg/mL（r = 0.999 8）

and 0.679 ～ 6.788 mg/mL（r =0.9995）

respectively. The average recoveries of eugenol

linalool

and eucalyptol were 100.34 %（RSD [relative standard deviation] = 1.18 %）

100.08 %（RSD = 1.32 %）

and 99.45 %（RSD = 1.47 %）

respectively. The content of eugenol

linalool

and eucalyptol in three batches of laurel oil was 12.73 ～ 14.17 mg/g

26.50 ～ 29.76 mg/g

and415.99 ～ 459.63 mg/g

respectively. Conclusion: The HPLC method is simple

rapid

accurate

and sensitive for quantifying eugenol

linalool

and eucalyptol in laurel oil with good repeatability and recovery

which can provide a scientific basis for the quality control and evaluation of laurel oil.