Objective: To analyze the distribution

drug resistance

and carbapenemase genotypes of commonGram-negative bacteria in bloodstream infection in our hospital

and to provide evidence for prevention and treat-ment of clinical bloodstream infection. Methods: We selected blood culture specimens examined between January2014 and December 2018

and took the isolated Gram-negative bacteria to identify the species and drug susceptibil-ity by the Mérieux ATB and antibiotic susceptibility testing system and determine the carbapenemase genotypes byPCR. Results: Among 13 028 blood culture samples examined

1 283 samples were positive

and Gram-negativebacteria

Gram-positive bacteria

and fungi were found in 58.07%（n = 745）

39.59%（n = 508）

and 2.34%（n =30） of the samples

respectively. The isolated bacteria mainly included Escherichia coli（61.07%

n = 480）

Klebsi-ella pneumoniae（18.19%

n = 143）

Enterobacter cloacae（4.71%

n = 37）

Pseudomonas aeruginosa（3.69%

n =29）

and Acinetobacter baumannii（2.42%

n = 19）. The resistance rates of the isolated bacteria to penicillins

ceph-alosporins

aminoglycosides

fluoroquinolones

sulfamethoxazole/trimethoprim

cephalosporins/enzyme inhibitors

and carbapenems were 85.44 ～ 100.00%

12.78 ～ 55.60%

0.00 ～ 8.30%

6.10 ～ 47.40%

13.57 ～ 96.00%

18.41 ～ 100.00%

and 0.00 ～ 100.00%

respectively. The carbapenemase-producing genes mainly included bla

KPC

bla

NDM

bla

IMP

and bla

OXA-23

and there were strains carrying both bla

KPC

and bla

NDM

. Conclusion:Gram-negative bacteria in bloodstream infection are severely resistant to common antibiotics. Clinical monitoring ofthe bacteria should be strengthened to guide rational drug use in clinical practice.