Significance of Notch1 and PTEN on bronchial asthma in children

Zhang Zhongxing

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Significance of Notch1 and PTEN on bronchial asthma in children

更新时间：2021-08-23

- Significance of Notch1 and PTEN on bronchial asthma in children
- Health Medicine Research and Practice Vol. 17, Issue 1, Pages: 55-58(2020)
- 作者机构：
  
  舞阳县人民医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R725.6
- Published：2020，
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Zhang Zhongxing. Significance of Notch1 and PTEN on bronchial asthma in children. [J]. Health Medicine Research and Practice 17(1):55-58(2020)
DOI：

Zhang Zhongxing. Significance of Notch1 and PTEN on bronchial asthma in children. [J]. Health Medicine Research and Practice 17(1):55-58(2020) DOI：

摘要

目的探讨Notch1和磷酸酯酶和张力蛋白同源物基因（PTEN）在儿童支气管哮喘中的作用及意义。方法选取2015年1月-2018年1月河南某医院诊治的41例支气管哮喘患儿（观察组）及同期在该院进行健康体检的40例儿童（对照组）作为研究对象。检测研究对象肺功能指标[一秒用力呼气容积（FEV1）、FEV1/用力肺活量（FVC）、用力呼气中期流速（FEF25%～75%）、用力呼气50%肺活量的瞬间流速（FEF50%）、用力呼气75%肺活量的瞬间流速（FEF75%）、呼出气一氧化氮（FeNO）]、白细胞介素-6（IL-6）和嗜酸性粒细胞（Eos）水平

qRT-PCR检测Notch1和PTEN mRNA表达水平

将所得数据进行统计分析。结果观察组研究对象的FEV1

FEV1/FVC

FEF25%～75%

FEF50%及FEF75%分别为（1.38±0.65）L

（60.35±10.42）%

（3.15±1.23）L/s

（1.76±0.79）L/s及（0.78±0.54）L/s

均低于对照组的（2.47±0.53）L

（79.46±13.26）%

（4.09±1.57）L/s

（2.57±1.03）L/s及（1.25±0.38）L/s（均P

0.05）。观察组研究对象的FeNO为（17.35±11.53）ppb

高于对照组的（10.46±4.25）ppb（P

0.05）。观察组研究对象的Eos及IL-6分别为（0.36±0.13）×109/L及（0.73±0.17）ng/mL

均高于对照组的（0.16±0.11）×109/L及（0.38±0.14）ng/mL（均P

0.05）。观察组研究对象的Notch1mRNA水平为1.52±0.13

明显高于对照组的0.87±0.09（P

0.05）;观察组研究对象的PTEN mRNA水平为0.38±0.08

明显低于对照组的1.17±0.19（P

0.05）。相关性分析结果显示

Notch1mRNA的表达水平与FEV1

FEV1/FVC

FEF25%～75%

FEF50%及FEF75%等指标呈现负相关（均P

0.05）

与FeNO

IL-6和Eos等指标呈正相关（均P

0.05）。而PTEN mRNA的表达水平与FEV

FEV

/FVC

FEF25%～75%

FEF50%及FEF75%等指标呈现正相关（均P

0.05）

与FeNO

IL-6和Eos呈负相关（均P

0.05）。且Notch1mRNA的表达与PTEN mRNA的表达呈现负相关（P

0.05）。结论哮喘患儿体内Notch1水平上调

Notch1可通过抑制PTEN表达及促进IL-6等炎症分子表达

诱导哮喘的发生和进展。

Abstract

Objective To investigate the significance of Notch1 and PTEN on bronchial asthma in children.Methods A total of 41 children with bronchial asthma（the study group）and 40 healthy children（the control group）in a hospital in Henan from January 2015 to January 2018 were selected as study objects.Indexes of pulmonary function[forced expiratory volume in one second（FEV1）

FEV1/forced vital capacity（FVC）

middle forced expiratory flow（FEF25%-75%）

forced expiratory flow at 50% of FVC（FEF50%）

forced expiratory flow at75% of FVC（FEF75%）and fractional exhaled nitric oxide（FeNO）]

interleukin-6（IL-6）and eosinophils（Eos）were detected;expression levels of Notch1 and PTEN mRNA were detected by qRT-PCR;all data were statistically analyzed.Results FEV1

FEV1/FVC

FEF25%-75%

FEF50%and FEF75%in the study group were（1.38±0.65）L

（60.35±10.42）%

（3.15±1.23）L/s

（1.76±0.79）L/s and（0.78±0.54）L/s

respectively.All were lower than those in the control group

which were（2.47±0.53）L

（79.46±13.26）%

（4.09±1.57）L/s

（2.57±1.03）L/s and（1.25±0.38）L/s

respectively（all P

0.05）.FeNO in the study group was（17.35±11.53）ppb

which was higher than that of（10.46±4.25）ppb in the control group（P

0.05）.Eos and IL-6 in the study group were（0.36±0.13）×109/L and（0.73±0.17）ng/mL

rspectively.Both were higher than those in the control group

which were（0.16±0.11）×109/L and（0.38±0.14）ng/mL

respectively（both P

0.05）.Level of Notch1 mRNA in the study group was 1.52±0.13

which was significantly higher than that of 0.87±0.09 in the control group（P

0.05）.Level of PTEN mRNA in the study group was 0.38±0.08

which was significantly lower than that of 1.17±0.19 in the control group（P

0.05）.The corresponding analysis results showed that expression level of Notch1 mRNA was negatively related with FEV

FEV

/FVC

FEF25%-75%

FEF50%and FEF75%（all P

0.05）

and was positively related with FeNO

IL-6 and Eos（all P

0.05）.Expression level of PTEN mRNA was positively related with FEV

FEV

/FVC

FEF25%-75%

FEF50% and FEF75%（all P

0.05）

and was negatively related with FeNO

IL-6 and Eos（all P

0.05）.Expression of Notch1 mRNA was negatively related with expression of PTEN mRNA（P

0.05）.Conclusion Upregulation of Notch1 level in children with asthma can suppress the expression of PTEN and promote the expression of inflammation factors like IL-6

so as to induce the development and progression of asthma.

关键词

Notch1PTEN儿童支气管哮喘炎症反应肺功能

Keywords

Notch1PTENchildrenBronchial asthmaInflammatory reactionPulmonary function

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