Objective To explore the way to establish a model of multiple human malignant melanoma metastases in nude mice so as to provide animal models for further study of the pathogenesis of malignant melanoma.Method Human melanoma cells MV3 were injected into dorsal subcutaneous tissues of nude mice.After tumors appeared in dorsal subcutaneous tissues

the animal models were detected with pathological examination and immunohistochemical techniques.Results 28 days later

nude mice successively had tumors in dorsal subcutaneous tissues after the injection.tumor pathological section immunohistochemistry showed that S-100 and HMB45were positive

tissue pathological section immunohistochemistry of HE staining

lung

spleen

liver

brain

and kidney showed that all these tissues had metastases to different degrees

which indicated the establishment of a model of multiple human malignant melanoma metastases in nude mice.Conclusion The study chooses human melanoma cells MV3 to inject into dorsal subcutaneous tissues of nude mice and after 28 days

a successful model of multiple human malignant melanoma metastases in nude mice is established.The animal model can provide an experimental evidence for further research of biological characteristics of melanoma and the development of new drugs.