Objective To analyze drug-resistance of Pseudomonas aeruginosa and the trend of its drug-resistance so as to provide the evidences for clinical prevention of infection and rational use of medicines.Method Specimens were collected by clinical departments with standard methods from January 2010to December 2012and were tested by using American BD BBL Crystal microbial identification instruments

the susceptibility were detected with the KirbyBauer disk diffusion Method（KB method）according to the standards of American Clinical and Laboratory Standards Institute（CLSI）.Results 316strains of Pseudomonas aeruginosa had the highest gentamicin-resistance with the rate of 69.4%and the lowest imipenem-resistance with the rate of 11.3% and the rest resistance rates for antimicrobial drugs tested were between 12.9%and 50.8%.Conclusion The drug-resistance of Pseudomonas aeruginosa is getting worse with a increasing trend year by year.Every hospital should strictly follow the rules of antimicrobial drug use and strengthen the surveillance and control of bacterial resistance.