OBJECTIVE

2

To analyze the differences in chemical components of

Gardenia jasminoides

before and after processing with ginger， and to evaluate the quality differences among different producing areas.

METHODS

2

Ultra-high performance liquid chromatography-tandem time-of-flight mass spectrometry was used to analyze the compositional differences of

G. jasminoides

before and after processing with ginger. The water content， total ash， and ethanol-soluble extract content of ginger-processed

G. jasminoides

were determined according to the 2020 edition of

Chinese Pharmacopoeia

. High performance liquid chromatography was adopted to determine the contents of genipin gentiobioside， geniposide， crocin Ⅰ and crocin Ⅱ in ginger-processed

G. jasminoides

.

RESULTS

2

A total of 49 chemical components were identified from raw

G. jasminoides

and ginger-processed

G. jasminoides

， including 14 flavonoids， 15 iridoids， 10 organic acids， 2 alkaloids and 8 other compounds. Among them， 42 components were detected in raw

G. jasminoides

， 28 in ginger-processed

G. jasminoides

， and 21 components were common to both. After processing with ginger， raw

G. jasminoides

lost 21 components （including iridoids， flavonoids， alkaloids， and others）， while 7 chemical components were added （including coumarins， organic acids， organic acid esters， and flavonoids）. For the 15 batches of ginger-processed

G. jasminoides

， the water content ranged from 5.64% to 7.11%， total ash from 2.92% to 4.87%， and ethanol-soluble extract from 40.61% to 58.02%. The average contents of genipin gentiobioside， geniposide， crocin Ⅰ and crocin Ⅱ were 0.108 7， 0.542 2， 0.565 0， and 0.012 5 mg/g， respectively.

CONCLUSIONS

2

After processing with ginger，

G. jasminoides

loses 21 components， while 7 new components are added. Differences are observed in the water content， total ash， ethanol-soluble extract， and the contents of genipin gentiobioside， geniposide， crocin Ⅰ， and crocin Ⅱ of ginger-processed

G. jasminoides

from different producing areas. Notably， samples from Fujian exhibit high contents of genipin gentiobioside and ethanol-soluble extract， while samples from Jiangxi have a high content of crocin Ⅰ.