OBJECTIVE

2

To compare the components of volatile oil from

Gardenia jasminoides

and their liver protective effect before and after stir-frying with wine.

METHODS

2

Steam distillation was used to exact the volatile oil from

G. jasminoides

and wine

stir-fried

G. jasminoides

. The components of volatile oil were identified by GC-MS method， and the relative mass fraction of each component was calculated by peak area normalization method. The rats were randomly divided into normal group， model group， positive control group （bifendate suspension 35 mg/kg），

G. jasminoides

low-dose and high-dose groups [1， 2 g/kg （calculated by crude drug）

]

and wine

stir-fried

G. jasminoides

low-dose and high-dose groups [1， 2 g/kg （calculated by crude drug）

]

with 10 rats in each group. Liver injury model was established by intraperitoneal injection of 40% carbon tetrachloride in rats of each group after continuous intragastric administration of corresponding drug solution for 7 days. The status， serum biochemical indexes， liver biochemical indexes and liver pathological sections of rats in each group were compared.

RESULTS

2

Twenty-three volatile oil components from

G. jasminoides

and 25 volatile oil components from wine

stir-fried

G. jasminoides

were identified； there were 18 common volatile oil components， of which the contents of 17 common components were decreased， while the content of one common component was increased due to stir-frying with wine. Compared with model group， the symptoms of depression and liver cell damage of rats in each administration group were improved to varying degrees； the s

erum levels of adenosine deaminase， alanine transaminase， aspartate transaminase， direct bilirubin， lactate dehydrogenase， prealbumin， total bile acid and total bilirubin were significantly decreased， while the total protein level was significantly increased； the level of malondialdehyde in liver tissue was significantly decreased， there were statistical significance （

P

＜0.05 or

P

＜0.01）.

CONCLUSIONS

2

During stir-frying with wine， the contents of 17 volatile oil components are decreased， while the content of one volatile oil component is increased. Wine

stir-fried

G. jasminoides

shows liver protective effect.

.