OBJECTIVE

2

To investigate the chemical composition difference of

Paeonia lactiflora

before and after being processed with wine and its analgesic effect.

METHODS

2

UPLC-Q-TOF-MS was used to analyze the chemical composition of

P. lactiflora

before and after being processed with wine. Wister rats were randomly divided into blank group， model group， positive control group （ibuprofen 0.072 mg/g） and

P. lactiflora

low-dose and high-dose groups （0.005， 0.01 mg/g， by raw mat

erial） and

P. lactiflora

stir-baked with wine low-dose and high-dose groups （0.005， 0.01 mg/g， by raw material）， with 6 rats in each group； the dysmenorrhea model of rats was induced by estradiol benzoate combined with oxytocin； the incubation period of body twisting in rats was recorded after the last medication； the contents of prostaglandin F

2α

（PGF

2α

）， progesterone， nitric oxide （NO） and

β-

endorphin in uterine tissue homogenate were detected.

RESULTS

2

Same 60 chemical compositions were identified in both positive and negative ion modes of

P. lactiflora

and

P. lactiflora

stir-baked with wine. The contents of 7 compositions， such as linalool， pedunculagin and syringic acid， were increased after stir-baked with wine， but the contents of other compositions were decreased. Compared with model group， the incubation period of body twisting in all administration groups （except for

P. lactiflora

low-dose group） was significantly prolonged （

P

＜0.05）； PGF

2α

in uterine tissue homogenate （except for

P. lactiflora

low-dose group） was significantly decreased； the contents of progesterone （except for

P. lactiflora

low-dose and high-dose groups）， NO （except for

P. lactiflora

low-dose and high-dose groups） and

β-

endorphin were significantly increased （

P

＜0.05）.

CONCLUSIONS

2

The analgesic effect of

P. lactiflora

stir-baked with wine on dysmenorrhea model rats is better than that of

P. lactiflora

， which may be related to the increase of 7 compositions such as linalool， pedunculagin and syringic acid after stir-baked with wine.