OBJECTIVE

2

To improve the quality standard for Jubei mixture.

METHODS

2

The chemical reaction of trinitrophenol and hydrocyanic acid was used to identify Armeniacae Amarum solution.

Platycodon grandiflorus

，

Fritillaria thunbergii

，

Scutellaria baicalensis

， the leaves of

Eriobotrya japonica

and

Glycyrrhiza uralensis

in Jubei mixture were identified by thin-layer chromatography （TLC）. High-performance liquid chromatography was used to simultaneously determine the contents of four active components， such as amygdalin， baicalin， wogonoside and monoammonium glycyrrhizinate in Jubei mixture.

RESULTS

2

Trinitrophenol test paper showed brick red. TLC spots of five medicinal materials were clear， without interference from negative control. The linear ranges of amygdalin， baicalin， wogonoside and monoammonium glycyrrhizinate were 0.066 8-1.335 5 μg （

R

2

＝0.9994）， 0.189 8-3.796 9 μg （

R

2

＝0.999 9）， 0.062 6-1.251 3 μg （

R

2

＝0.999 9）， 0.041 8-0.835 5 μg （

R

2

＝0.999 8）， respectively. RSDs of precision， repeatability and stability tests were all lower than 2%. The average recoveries were 98.2%， 99.5%， 98.3%， 99.6%， and RSDs were 0.7%， 1.6%， 1.5%， 1.1% （

n

＝6）. The contents of 15 batches of samples were different， and the content of sample from company A was generally lower than those from companies B and C.

CONCLUSIONS

2

The standard established can reflect the inherent quality of Jubei mixture well.